Absence of donor-derived zona pellucida protein C homolog in the inner perivitelline layer of Peking duck (Anas platyrhynchos)-Japanese quail (Coturnix coturnix japonica) chimeras (Duails)
| Autor: | G. Tao, J. Sha, Jing Gao, C. J. Zhao, Haitang Han, Qingna Zhao, J. Y. Li, Zonggang Li, Makoto Mori |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Male
medicine.medical_specialty animal structures Acrosome reaction Molecular Sequence Data Receptors Cell Surface Coturnix Biology Polymerase Chain Reaction Zona Pellucida Glycoproteins Chimera (genetics) Internal medicine biology.animal medicine Sexual maturity Animals Amino Acid Sequence Zona pellucida Crosses Genetic DNA Primers chemistry.chemical_classification Membrane Glycoproteins Chimera Egg Proteins Embryo General Medicine biology.organism_classification Molecular biology Quail Peptide Fragments Amino acid medicine.anatomical_structure Endocrinology Ducks chemistry Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization embryonic structures Coturnix coturnix Animal Science and Zoology Female |
| Zdroj: | Poultry science. 87(10) |
| ISSN: | 0032-5791 |
| Popis: | Avian blastodermal cells at stage X are used to produce interspecies chimeras for heterogenous poultry reproduction. However, recipient-derived inner perivitelline layer (IPVL)-enclosed donor-derived ova may affect the efficiency of germline transmission via chimera. Among the proteins in the IPVL, zona pellucida protein C (ZPC) plays an important role in sperm-egg binding and inducing the acrosome reaction. In the present study, Peking duck blastodermal cells at stage X were transferred into subgerminal cavities of Japanese quail embryos at the same stage. Fourteen female duck-quail chimeras (duails) were hatched and raised to sexual maturity. After being screened by PCR, 3 duails were selected for examination of donor-derived ZPC. A total of 152 IPVL protein samples from the individual eggs laid by the 3 duails then underwent a preliminary examination for the presence of donor-derived ZPC by means of SDS-PAGE, periodic acid-Schiff staining, and Western blotting. A novel 35-kDa ZPC, not observed in quail but in duck, was found in the IPVL of the duails. Further analysis of peptide mass fingerprinting of Peking duck ZPC, Japanese quail ZPC, and the 35-kDa duail ZPC by matrix-assisted laser desorption-ionization reflectron time-of-flight mass spectrometry revealed that the novel ZPC was an isoform of quail ZPC. Moreover, comparison of N-terminal amino acid sequences of these 3 ZPC confirmed that the 35-kDa quail ZPC had more amino acids at the N terminus than did native quail ZPC, and none of the donor-derived ZPC was found in the duails. These findings suggest that it would be difficult to obtain donor-derived offspring by natural mating of interspecies chimeras. |
| Databáze: | OpenAIRE |
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