Association of MBP peptides with Hsp70 in normal appearing human white matter
| Autor: | Michael W. Jakowec, Lilit Mnatsakanyan, Brett T. Lund, Nazely Ashikian, Rodrigo Aguilera, Yervand Chakryan, Carolyn Bevan, Tim Gallaher |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Multiple Sclerosis
T-Lymphocytes Molecular Sequence Data Epitopes T-Lymphocyte Nerve Tissue Proteins T-Cell Antigen Receptor Specificity Biology medicine.disease_cause Autoantigens Cell Line Autoimmunity White matter Myelin Immune system Adjuvants Immunologic Heat shock protein Immunopathology Protein Interaction Mapping medicine Humans Immunoprecipitation HSP70 Heat-Shock Proteins Amino Acid Sequence Myelin Sheath Brain Chemistry Autoimmune disease Immunodominant Epitopes Multiple sclerosis Myelin Basic Protein medicine.disease Peptide Fragments medicine.anatomical_structure Neurology Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Immunology Neurology (clinical) Transcription Factors |
| Zdroj: | Journal of the Neurological Sciences. 249:122-134 |
| ISSN: | 0022-510X |
| Popis: | Multiple Sclerosis is an autoimmune disease directed against myelin proteins. The etiology of MS is poorly defined though, with no definitive causative agent yet identified. It has been hypothesized that MS may be a multifactorial disease resulting in the same end product: the destruction of myelin by the immune system. In this report we describe a potential role for heat shock proteins in the pathogenesis of MS. We isolated Hsp70 from the normal appearing white matter of both MS and normal human brain and found this was actively associated with, among other things, immunodominant MBP peptides. Hsp70-MBP peptide complexes prepared in vitro were shown to be highly immunogenic, with adjuvant-like effects stimulating MBP peptide-specific T cell lines to respond to normally sub-optimal concentrations of peptide. This demonstration of a specific interaction between Hsp70 and different MBP peptides, coupled with the adjuvanticity of this association is suggestive of a possible role for Hsp70 in the immunopathology associated with MS. |
| Databáze: | OpenAIRE |
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