Complete genome sequence of a non-pathogenic strain of Fowl Adenovirus serotype 11: Minimal genomic differences between pathogenic and non-pathogenic viruses
| Autor: | Sara M. Uribe-Ochoa, Eduardo Lucio-Decanini, Diana V. Cortés-Espinosa, Andrés Morales-Garzón, Laura J. García, Angel E. Absalón, Pedro F. Vera-Hernández |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Serotype 040301 veterinary sciences Adenoviridae Infections Molecular Sequence Data Virulence Genome Viral Biology Genome Virus law.invention 0403 veterinary science 03 medical and health sciences Open Reading Frames Viral Proteins law Virology Animals Amino Acid Sequence ORFS Polymerase chain reaction Poultry Diseases Whole genome sequencing Base Sequence Aviadenovirus 04 agricultural and veterinary sciences Open reading frame 030104 developmental biology Chickens Sequence Alignment |
| Zdroj: | Virology. 501 |
| ISSN: | 1096-0341 |
| Popis: | In this study, we conducted the clinicopathological characterization of a non-pathogenic FAdV-D serotype 11 strain MX95, isolated from healthy chickens, and its entire genome was sequenced. Experiments in SPF chickens revealed that the strain is a non-pathogenic virus that did not cause death at challenge doses of 1×106 TCID50. Additionally, the infection in SPF chickens caused no apparent damage in most of the organs analyzed by necropsy and histopathology, but it did cause inclusion body hepatitis; nevertheless it did not generate severe infectious clinical symptoms. The virus was detected in several chicken organs, including the lymphoid organs, by real-time polymerase chain reaction (PCR) until 42 days. The genome of FAdV-11 MX95 has a size of 44,326bp, and it encodes 36 open reading frames (ORFs). Comparative analysis of the genome indicated only 0.8% dissimilarity with a highly virulent serotype 11 that was previously reported. |
| Databáze: | OpenAIRE |
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