The MAGUK Protein MPP7 Binds to the Polarity Protein hDlg1 and Facilitates Epithelial Tight Junction Formation
| Autor: | Kris Gevaert, Volker M. Stucke, Joël Vandekerckhove, Evy Timmerman, Alan Hall |
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| Přispěvatelé: | UCL |
| Rok vydání: | 2007 |
| Předmět: |
DNA
Complementary Polarity (physics) Molecular Sequence Data Plasma protein binding Biology Transfection Cell Line Tight Junctions Discs Large Homolog 1 Protein Adherens junction Cell polarity Humans Amino Acid Sequence Molecular Biology Adaptor Proteins Signal Transducing Epithelial polarity Membrane Glycoproteins Base Sequence Tight junction Cell Polarity Membrane Proteins Epithelial Cells Articles Cell Biology Recombinant Proteins Protein Structure Tertiary Cell biology Membrane protein Caco-2 RNA Interference Caco-2 Cells HeLa Cells Protein Binding |
| Zdroj: | Molecular Biology of the Cell, Vol. 18, no. 5, p. 1744-1755 (2007) |
| ISSN: | 1939-4586 1059-1524 |
| Popis: | Three groups of evolutionarily conserved proteins have been implicated in the establishment of epithelial cell polarity: the apically-localized proteins of the Par (Par3-Par6-aPKC-Cdc42) and Crumbs groups (Crb3-PALS1-PATJ) and the basolaterally localized proteins of the Dlg group (Dlg1-Scribble-Lgl). During epithelial morphogenesis, these proteins participate in a complex network of interdependent interactions that define the position and functional organization of adherens junctions and tight junctions. However, the biochemical pathways through which they control polarity are poorly understood. In this study, we identify an interaction between endogenous hDlg1 and MPP7, a previously uncharacterized MAGUK-p55 subfamily member. We find that MPP7 targets to the lateral surface of epithelial cells via its L27N domain, through an interaction with hDlg1. Loss of either hDlg1 or MPP7 from epithelial Caco-2 cells results in a significant defect in the assembly and maintenance of functional tight junctions. We conclude that the formation of a complex between hDlg1 and MPP7 promotes epithelial cell polarity and tight junction formation. |
| Databáze: | OpenAIRE |
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