Distribution of Signaling Molecules Involved in Vasopressin-induced Ca2+ Mobilization in Rat Hepatocyte Multiplets
| Autor: | Thierry Durroux, Michel Claret, Dien Tran, Gilles Guillon, Arlette Forchioni, Marie Noelle Dufour, Nicole Stelly, René Seyer, Thierry Tordjmann |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Phosphatidylinositol 4
5-Diphosphate 0301 basic medicine Receptors Vasopressin Cell signaling Vasopressin Histology Vasopressins Phospholipase C beta Inositol 1 4 5-Trisphosphate law.invention 03 medical and health sciences GTP-Binding Proteins Tubulin Confocal microscopy law medicine Animals Calcium Signaling Rats Wistar Receptor Cytoskeleton Cells Cultured 030102 biochemistry & molecular biology Chemistry Antagonist Fluorescence Molecular biology Actins Rats Cell biology Isoenzymes 030104 developmental biology medicine.anatomical_structure Liver Microscopy Fluorescence Type C Phospholipases Hepatocyte GTP-Binding Protein alpha Subunits Gq-G11 Female Anatomy Subcellular Fractions |
| Zdroj: | Journal of Histochemistry & Cytochemistry. 47:601-616 |
| ISSN: | 1551-5044 0022-1554 |
| DOI: | 10.1177/002215549904700503 |
| Popis: | In freshly isolated rat hepatocyte multiplets, Ca2+ signals in response to vasopressin are highly organized. In this study we used specific probes to visualize, by fluorescence and confocal microscopy, the main signaling molecules involved in vasopressin-mediated Ca2+ responses. V1a receptors were detected with a novel fluorescent antagonist, Rhm8 -PVA. The Gαq/Gα11, PLCβ3, PIP2, and InsP3 receptors were detected with specific antibodies. V1a vasopressin receptors and PIP2 were associated with the basolateral membrane and were not detected in the bile canalicular domain. Gαq/Gα11, PLCβ3, and InsP3 receptors were associated with the basolateral membrane and also with other intracellular structures. We used double labeling, Western blotting, and drugs (cytochalasin D, colchicine) known to disorganize the cytoskeleton to demonstrate the partial co-localization of Gαq/Gα11 with F-actin. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|