Radiation‐induced variations in urothelial expression of intercellular adhesion molecule 1 (ICAM‐1): association with changes in urinary bladder function
| Autor: | J Jaal, Kerstin Brüchner, Wolfgang Dörr, C Hoinkis |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Pathology
medicine.medical_specialty Statistics as Topic Urinary Bladder Intercellular Adhesion Molecule-1 Urology Radiation Dosage Mice medicine Animals Radiology Nuclear Medicine and imaging Irradiation Urothelium Mice Inbred C3H ICAM-1 Urinary bladder Radiological and Ultrasound Technology Chemistry Dose-Response Relationship Radiation Staining Dose–response relationship medicine.anatomical_structure Immunohistochemistry Female |
| Zdroj: | International Journal of Radiation Biology. 80:65-72 |
| ISSN: | 1362-3095 0955-3002 |
| DOI: | 10.1080/09553000310001632921 |
| Popis: | To assess the effect of single-dose irradiation on intercellular adhesion molecule 1 (ICAM-1) expression in the urothelium of mouse urinary bladder and to correlate ICAM-1 variations with fluctuations in storage capacity during the early and late radiation response.Groups of female C3H mice were subjected to irradiation with either 20 or 0 Gy. The intensity of immunohistochemical ICAM-1 staining in the urothelium was assessed in a semiquantitative way applying an arbitrary score (0-5). Changes in bladder storage function were assessed by transurethral cystometry.For the early radiation response phase, a reduction in bladder capacity by50%, i.e. a positive functional radiation response, was seen in 40% of the irradiated animals between days 0 and 15, and in 64% of animals during days 16-30. During the late response phase, 71% of the animals sacrificed after day 180 developed a positive functional response. Urothelial cells were found to express ICAM-1 constitutively. Irradiation resulted in an early rise in staining signal by day 2, with a maximum on day 4 and a return to control values on day 13. A permanent increase in ICAM-1 staining signal was observed in the late phase, from day 90 to 360 after irradiation. The expression of ICAM-1 in animals with a positive late response was 4.2+/-1.2 (mean+/-standard deviation), compared with 2.6+/-1.0 in non-responders (p=0.0009).Irradiation induces significant acute and chronic changes in urothelial ICAM-1 expression indicating that the urothelium contributes to the pathogenesis of both acute and late radiation effects in the urinary bladder. |
| Databáze: | OpenAIRE |
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