Characterization and cytocompatibility of 3D porous biomimetic scaffold derived from rabbit nucleus pulposus tissue in vitro

Autor: Mingxin Wu, Jin Sun, Chu-Song Zhou, Yu Zhang, You Wu, Wei Tan, Wei Cao
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Materials science
Nucleus Pulposus
Cell Survival
Tissue Engineering Constructs and Cell Substrates
Biomedical Engineering
Biophysics
Cell Culture Techniques
Tetrazolium Salts
Bioengineering
Biocompatible Materials
Bone Marrow Cells
02 engineering and technology
Biomaterials
Extracellular matrix
03 medical and health sciences
Tissue engineering
Biomimetics
medicine
Pressure
Animals
030304 developmental biology
Cell Proliferation
0303 health sciences
Decellularization
Tissue Engineering
Tissue Scaffolds
Regeneration (biology)
Biomaterial
Intervertebral disc
Mesenchymal Stem Cells
Adhesion
021001 nanoscience & nanotechnology
In vitro
Extracellular Matrix
Thiazoles
medicine.anatomical_structure
Microscopy
Fluorescence
Microscopy
Electron
Scanning
Rabbits
Stress
Mechanical
0210 nano-technology
Porosity
Biomedical engineering
Zdroj: Journal of Materials Science. Materials in Medicine
ISSN: 1573-4838
0957-4530
Popis: Intervertebral disc (IVD) degeneration is one of the most important causes of lower back pain. Tissue engineering provides a new method for the experimental treatment of degenerative disc diseases. This study aims to develop a natural, acellular, 3D interconnected porous scaffold derived from the extracellular matrix (ECM) of nucleus pulposus. The nucleus pulposus (NP) was decellularized by sequential detergent-nuclease methods, including physical crushing, freeze-drying and cross-linking. These 3D porous scaffolds were fabricated with a high porosity of (81.28 ± 4.10)%, an ideal pore size with appropriate mechanical properties. Rabbit bone marrow mesenchymal stem cells (rBMSCs) were seeded and cultured on the scaffolds. And the mechanical tests showed the compressive elastic modulus of the scaffolds cultured for 4 weeks reached 0.12 MPa, which was better than that of the scaffolds cultured for 2 weeks (0.07 MPa) and that of the control group (0.04 MPa). Scanning electron microscopy (SEM), histological assays, molecular biology assays revealed that the scaffolds could provide an appropriate microstructure and environment for the adhesion, proliferation, migration and secretion of seeded cells in vitro. As assays like histology, immunohistochemistry and the real-time qRT-PCR showed, NP-like tissues were preliminarily formed. In conclusion, the 3D porous scaffold derived from NP ECM is a potential biomaterial for the regeneration of NP tissues. A natural, acellular, 3D interconnected porous scaffold derived from the extracellular matrix (ECM) of nucleus pulposus was developed by sequential detergent-nuclease and freeze-drying method, which can reduce the damage of protein activity to the minimum. It is very similar to the composition and internal environment of the natural nucleus pulposus, because it derived from the natural nucleus pulposus. Scanning electron microscopy (SEM), histological assays, molecular biology assays revealed that the scaffolds could provide an appropriate microstructure and environment for the adhesion, proliferation, migration, and secretion of seeded cells in vitro.
Databáze: OpenAIRE
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