Synthetic enzyme-based nanoparticles act as smart catalyst for glucose responsive release of insulin
| Autor: | Baishali A. Jana, Ujwala A Shinde, Ashish Wadhwani |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine medicine.medical_treatment Nanoparticle Bioengineering 01 natural sciences Applied Microbiology and Biotechnology Glucose Oxidase 03 medical and health sciences chemistry.chemical_compound Drug Delivery Systems 010608 biotechnology Diabetes mellitus Hyaluronic acid medicine Humans Insulin Glucose oxidase chemistry.chemical_classification biology Artificial enzyme General Medicine medicine.disease Glucose 030104 developmental biology Enzyme Biochemistry chemistry Drug delivery biology.protein Nanoparticles Biotechnology |
| Zdroj: | Journal of Biotechnology. 324:1-6 |
| ISSN: | 0168-1656 |
| DOI: | 10.1016/j.jbiotec.2020.09.023 |
| Popis: | Background Earlier studies have attempted to create electronic free insulin delivery systems using different glucose sensing mechanism, no successful clinical translation as hitherto been made. This study aimed to assess the faster responsiveness of the insulin release from this enzyme based nanoparticles which is a self-regulated insulin delivery system constructed by loading with insulin, enzyme glucose oxidase into hyaluronic acid and 2-nitroimidazole forming enzyme-based nanoparticles which works in accordance to the blood glucose level. Materials and method Enzyme-based nanoparticles were prepared by ionic gelation method. Insulin content in the nanoparticles kept for stability study was estimated by human insulin enzyme based immunosorbent assay. In in-vitro studies; different concentrations of glucose were taken and the release study of insulin was recorded. Results This enzyme-based nanoparticles were having average diameter of around 193 nm and stability studies showed that nanoparticles were stable upto 30 days at 4 °C. In-vitro studies showed the release of insulin from nanoparticle conjugates which was effectively correlated with the external glucose concentration created where different concentrations of glucose taken thus facilitating the stabilization of blood glucose levels in the hyperglycemia state which was achieved within 10 min. (400 mg/dL) wherein drug release rate remarkably increased in hyperglycemia state and no specific changes or small amount of release was observed in normoglycemia state (100 mg/dL). Conclusion Overall, this preliminary study of this enzyme-based nanoparticles formulation showed excellent rapid responsiveness towards hyperglycemia which might act as a potential biomimetic system in triggering the release of insulin in sustained manner. |
| Databáze: | OpenAIRE |
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