Production of d-xylonic acid using a non-recombinant Corynebacterium glutamicum strain
| Autor: | Andreas Radek, Pornkamol Unrean, Niklas Tenhaef, Stephan Noack, René Hilmes, Christian Brüsseler, Jan Marienhagen |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Environmental Engineering Xylose Renewable Energy Sustainability and the Environment 030106 microbiology Lignocellulosic biomass Sugar Acids Bioengineering Dehydrogenase General Medicine Xylonic acid Corynebacterium glutamicum 03 medical and health sciences chemistry.chemical_compound Hydrolysis 030104 developmental biology Glucose chemistry Biochemistry Fermentation Bioprocess Waste Management and Disposal |
| Zdroj: | Bioresource technology. 268 |
| ISSN: | 1873-2976 |
| Popis: | It was found that Corynebacterium glutamicum ΔiolR devoid of the transcriptional regulator IolR accumulates high amounts of d -xylonate when cultivated in the presence of d -xylose. Detailed analyses of constructed deletion mutants revealed that the putative myo-inositol 2-dehydrogenase IolG also acts as d -xylose dehydrogenase and is mainly responsible for d -xylonate oxidation in this organism. Process development for d -xylonate production was initiated by cultivating C. glutamicum ΔiolR on defined d -xylose/ d -glucose mixtures under batch and fed-batch conditions. The resulting yield matched the theoretical maximum of 1 mol mol−1 and high volumetric productivities of up to 4 g L−1 h−1 could be achieved. Subsequently, a novel one-pot sequential hydrolysis and fermentation process based on optimized medium containing hydrolyzed sugarcane bagasse was developed. Cost-efficiency and abundance of second-generation substrates, good performance indicators, and enhanced market access using a non-recombinant strain open the perspective for a commercially viable bioprocess for d -xylonate production in the near future. |
| Databáze: | OpenAIRE |
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