Functional Heterogeneity of Protein Kinase A Activation in Multipotent Stromal Cells
| Autor: | Natalia Kalinina, Konstantin Y. Kulebyakin, Vadim I Chechekhin, Pyotr A. Tyurin-Kuzmin, Veronika Yu. Sysoeva, Anastasiya M Ivanova, Mariya N Skryabina, Maxim Karagyaur, Daniyar T. Dyikanov, Vsevolod A. Tkachuk, Mikhail Arbatskiy |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Stromal cell
single cell analysis Clonal analysis Article Gene Expression Regulation Enzymologic Catalysis Inorganic Chemistry lcsh:Chemistry Protein kinase A activation Single-cell analysis Cyclic AMP Humans multipotent stromal cells Physical and Theoretical Chemistry Protein kinase A mesenchymal stem/stromal cells Molecular Biology lcsh:QH301-705.5 Cells Cultured Spectroscopy Chemistry Organic Chemistry Mesenchymal stem cell intracellular signaling protein kinase A functional heterogeneity PKA-Spark biosensor Mesenchymal Stem Cells General Medicine Adenylyl Cyclases Cyclic AMP-Dependent Protein Kinases Hormones Computer Science Applications Cell biology lcsh:Biology (General) lcsh:QD1-999 Signal Transduction Hormone |
| Zdroj: | International Journal of Molecular Sciences; Volume 21; Issue 12; Pages: 4442 International Journal of Molecular Sciences, Vol 21, Iss 4442, p 4442 (2020) International Journal of Molecular Sciences |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms21124442 |
| Popis: | Multipotent stromal cells (MSC) demonstrate remarkable functional heterogeneity; however, its molecular mechanisms remain largely obscure. In this study, we explored MSC response to hormones, which activate Gs-protein / cyclic AMP (cAMP) / protein kinase A (PKA) dependent signaling, at the single cell level using genetically encoded biosensor PKA-Spark. For the first time, we demonstrated that about half of cultured MSCs are not able to activate the cAMP/PKA pathway, possibly due to the limited availability of adenylyl cyclases. Using this approach, we showed that MSC subpopulations responding to various hormones largely overlapped, and the share of responding cells did not exceed 40%. Using clonal analysis, we showed that signaling heterogeneity of MSC could be formed de novo within 2 weeks. |
| Databáze: | OpenAIRE |
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