Immunoglobulin recognition of fecal bacteria in stunted and non-stunted children: findings from the Afribiota study

Autor: Pascale Vonaesch, Nathalie Kapel, Michel Dede, André Rodriguez-Pozo, Amee R. Manges, Alison Nestoret, Afribiota Investigators, Azimdine Habib, B. Brett Finlay, Philippe J. Sansonetti, Kelsey E. Huus, Jean-Marc Collard
Přispěvatelé: University of British Columbia (UBC), Pathogénie microbienne moléculaire, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Unité des Helminthiases [Antananarivo, Madagascar] (IPM), Institut Pasteur de Madagascar, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Institut Pasteur de Bangui, Réseau International des Instituts Pasteur (RIIP), Unité de Bactériologie Expérimentale [Antananarivo, Madagascar] (IPM), Chaire Microbiologie et Maladies infectieuses, Collège de France (CdF (institution)), This research was supported by grants from the Canadian Institutes of Health Research (CIHR), the Bill and Melinda Gates Foundation and the Total Foundation. Work in PJS’s laboratory is supported by grants from the European Research Commission. Work in BBF’s laboratory was supported by grants from CIHR. KEH was supported by a CIHR Vanier Graduate scholarship, a Killam fellowship and a Four-Year Fellowship. PV was supported by a Swiss National Science Foundation Early and Advanced Postdoctoral Fellowship as well as Return Grant, a Roux-Cantarini Postdoctoral Fellowship and a fellowship from L’Oréal-UNESCO for Women in Science., We are deeply grateful to the children and families who participated in Afribiota and to the large team of community health workers, nurses, doctors, technicians, administrators, and others without whom this study would not have been possible. We would like to thank Andy Johnson and Justin Wong at UBC for their invaluable assistance in the flow cytometry facility, as well as Lisa Thorson and Adrian Carney for their help in arranging and receiving sample shipments at the Michael Smith Laboratories. We are further grateful to Vincent Guillemot and Antoine Menard at the Institut Pasteur for their advice on biostatistic analysis and to the members of the Sansonetti research group and the Finlay laboratory for their support and feedback., The Afribiota Investigators are: (in alphabetical order): Emilson Jean Andriatahirintsoa, Alexandra Bastaraud, Jean-Marc Collard, Maria Doria, Serge Ghislain Djorie, Aurélie Etienne, Brett Finlay, Tamara Giles-Vernick, Jean-Chrysostome Gody, Bolmbaye Privat Godje, Ionela Gouandjika-Vassilache, Francis Allan Hunald, Nathalie Kapel, Jean-Pierre Lombart, Alexandre Manirakiza, Synthia Nazita Nigatoloum, Lisette Raharimalala, Maheninasy Rakotondrainipiana, Rindra Randremanana, Harifetra Mamy Richard Randriamizao, Frédérique Randrianirina, Annick Robinson, Pierre-Alain Rubbo, Philippe Sansonetti, Laura Schaeffer, Inès Vigan-Womas, Sonia Sandrine Vondo, Pascale Vonaesch & Laura Wegener-Parfrey, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Collège de France - Chaire Microbiologie et Maladies infectieuses
Rok vydání: 2020
Předmět:
Male
Immunoglobulin A
medicine.disease_cause
Immunoglobulin G
Feces
Medical microbiology
RNA
Ribosomal
16S
Growth Disorders
2. Zero hunger
Stunting
0303 health sciences
Sub-Saharan Africa
biology
Campylobacter
3. Good health
Central African Republic
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
Child
Preschool
Environmental Enteric dysfunction
lcsh:QR100-130
[SDV.IMM]Life Sciences [q-bio]/Immunology
Female
Microbiology (medical)
medicine.medical_specialty
[SDV.BC]Life Sciences [q-bio]/Cellular Biology
Microbiology
lcsh:Microbial ecology
03 medical and health sciences
Haemophilus
Madagascar
medicine
Humans
Microbiome
030304 developmental biology
Bacteria
030306 microbiology
Research
Malnutrition
Undernutrition
[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Molecular biology
biology.organism_classification
medicine.disease
Fecal coliform
Immunology
biology.protein
[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie
Zdroj: Microbiome
Microbiome, BioMed Central, 2020, 8, pp.113. ⟨10.1186/s40168-020-00890-1⟩
Microbiome, Vol 8, Iss 1, Pp 1-16 (2020)
Microbiome, 2020, 8, pp.113. ⟨10.1186/s40168-020-00890-1⟩
ISSN: 2049-2618
DOI: 10.1186/s40168-020-00890-1
Popis: Background Child undernutrition is a global health issue that is associated with poor sanitation and an altered intestinal microbiota. Immunoglobulin (Ig) A mediates host-microbial homeostasis in the intestine, and acutely undernourished children have been shown to have altered IgA recognition of the fecal microbiota. We sought to determine whether chronic undernutrition (stunting) or intestinal inflammation were associated with antibody recognition of the microbiota using two geographically distinct populations from the Afribiota project. Fecal bacteria from 200 children between 2 and 5 years old in Antananarivo, Madagascar, and Bangui, Central African Republic (CAR), were sorted into IgA-positive (IgA+) and IgA-negative (IgA−) populations by flow cytometry and subsequently characterized by 16S rRNA gene sequencing to determine IgA-bacterial targeting. We additionally measured IgG+ fecal bacteria by flow cytometry in a subset of 75 children. Results Stunted children (height-for-age z-score ≤ −2) had a greater proportion of IgA+ bacteria in the fecal microbiota compared to non-stunted controls. This trend was consistent in both countries, despite the higher overall IgA-targeting of the microbiota in Madagascar, but lost significance in each country individually. Two of the most highly IgA-recognized bacteria regardless of nutritional status were Campylobacter (in CAR) and Haemophilus (in both countries), both of which were previously shown to be more abundant in stunted children; however, there was no association between IgA-targeting of these bacteria and either stunting or inflammatory markers. IgG-bound intestinal bacteria were rare in both stunted and non-stunted children, similar to levels observed in healthy populations. Conclusions Undernourished children carry a high load of intestinal pathogens and pathobionts. Our data suggest that stunted children have a greater proportion of IgA-recognized fecal bacteria. We moreover identify two putative pathobionts, Haemophilus and Campylobacter, that are broadly targeted by intestinal IgA. This study furthers our understanding of host-microbiota interactions in undernutrition and identifies immune-recognized microbes for future study.
Databáze: OpenAIRE
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