NSF is required for the brefeldin A-promoted disassembly of the Golgi apparatus
| Autor: | Mitsuo Tagaya, Akitsugu Yamamoto, Katsuko Tani, Takuya Fukunaga, Akiko Furuno, Kiyotaka Hatsuzawa |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Retrograde transport
Protein subunit Biophysics Vesicular Transport Proteins Golgi Apparatus CHO Cells Cyclopentanes Biology Biochemistry symbols.namesake chemistry.chemical_compound Structural Biology Cricetinae Genetics Animals Molecular Biology Microinjection N-Ethylmaleimide-Sensitive Proteins Protein Synthesis Inhibitors Brefeldin A Cell Biology COPI Golgi apparatus Cell biology Transport protein Anti-Bacterial Agents Microscopy Electron chemistry Golgi disassembly symbols Axoplasmic transport Macrolides Carrier Proteins N-Ethylmaleimide-sensitive factor |
| Zdroj: | FEBS letters. 435(2-3) |
| ISSN: | 0014-5793 |
| Popis: | N-Ethylmaleimide-sensitive factor (NSF) is required for multiple pathways of vesicle-mediated protein transport. Microinjection of a monoclonal anti-NSF antibody almost completely blocked brefeldin A-promoted Golgi disassembly without affecting the rapid release of β-COP, a subunit of the Golgi coat proteins (COPI), from the Golgi apparatus. Similar results were obtained using a dominant-negative NSF which is known to compete with endogenous NSF. The present results suggest that an NSF-mediated step is present in the brefeldin A-promoted disassembly of the Golgi apparatus. |
| Databáze: | OpenAIRE |
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