Heterologous expression of pyruvate dehydrogenase E1 subunits of the microsporidiumParanosema(Antonospora)locustaeand immunolocalization of the mitochondrial protein in amitochondrial cells
| Autor: | Elena V. Seliverstova, Igor V. Senderskiy, Anton M. Naumov, Galina V. Beznoussenko, Viacheslav V. Dolgikh, Olga A. Pavlova |
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| Rok vydání: | 2009 |
| Předmět: |
Immunoelectron microscopy
Respiratory chain Mitochondrion Biology Microbiology Fungal Proteins Mitochondrial Proteins Western blot Escherichia coli Genetics medicine Immunoprecipitation Pyruvate Dehydrogenase (Lipoamide) Microscopy Immunoelectron Molecular Biology medicine.diagnostic_test Cryoelectron Microscopy Spores Fungal Pyruvate dehydrogenase complex Molecular biology Recombinant Proteins Citric acid cycle Protein Subunits Biochemistry Cytoplasm Apansporoblastina Protein Binding |
| Zdroj: | FEMS Microbiology Letters. 293:285-291 |
| ISSN: | 1574-6968 0378-1097 |
| DOI: | 10.1111/j.1574-6968.2009.01545.x |
| Popis: | Microsporidia, a large group of fungi-related intracellular parasites, are characterized by drastically reduced metabolism. They possess genes encoding glycolysis components, and the glycerol-phosphate shuttle, but lack mitochondria, Krebs cycle, respiratory chain and pyruvate-converting enzymes, except alpha and beta subunits of E(1) enzyme of pyruvate dehydrogenase (PDH) complex. Here, we have expressed PDH subunits from the microsporidum Paranosema (Antonospora) locustae in Escherichia coli. Western blot analysis with antibodies raised against recombinant proteins has revealed their specific accumulation in mature spores of P. locustae but not in the intracellular development stages. Two subunits were coprecipitated as a single heterooligomeric complex by anti-alpha or anti-beta PDH antibodies. Ultracentrifugation of spore homogenate has shown the presence of PDH in the soluble fraction. Relocalization of the mitochondrial protein in microsporidial spore cytoplasm was confirmed by immunoelectron microscopy of ultrathin cryosections with affinity-purified anti-alpha PDH antibodies. On cryosections, parasite enzyme was found partly associated with the cytoplasmic side of ER and other intraspore membranes, suggesting that electrons might be transferred to any membrane acceptor and finally to oxygen in the parasite cell. |
| Databáze: | OpenAIRE |
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