A nationwide multicentre study in Turkey for establishing reference intervals of haematological parameters with novel use of a panel of whole blood
| Autor: | Özgül Gözlükaya, Yuksel Aliyazicioglu, Gulcin Eskandari, Yesim Guvenc, Okhan Akin, Ebubekir Bakan, Pinar Eker, Melahat Dirican, Fatma Gun, Fatma Taneli, Harun Polat, Meltem Demir, Murat Örmen, Nuriye Mete, Nurten Aksoy, Ozlem Ceylan, Gul Ozlem Tuncer, Nurinnisa Ozturk, Tevfik Noyan, Gürbüz Polat, Sevim Kahraman, Hatice Yüksel, Hatice Sezen, Shogo Kimura, Yesim Ozarda, Nurcan Kilic Baygutalp, Kiyoshi Ichihara, Husamettin Vatansev, Zübeyde Erbayraktar |
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| Přispěvatelé: | Selçuk Üniversitesi |
| Rok vydání: | 2017 |
| Předmět: |
Adult
Male Quality Control Laboratory Proficiency Testing 030213 general clinical medicine medicine.medical_specialty Pediatrics complete blood count Adolescent Turkey Clinical Biochemistry 030204 cardiovascular system & hematology Gastroenterology Young Adult 03 medical and health sciences 0302 clinical medicine Reference Values Internal medicine Healthy volunteers medicine Humans Aged Whole blood multicentre study reference intervals haematology Hematologic Tests Hematology medicine.diagnostic_test biology business.industry Biochemistry (medical) Reproducibility of Results Complete blood count Middle Aged Original Papers Blood Cell Count Reference intervals Ferritin Reference values biology.protein Regression Analysis Female Laboratories business Regional differences |
| Zdroj: | Biochemia Medica Volume 27 Issue 2 |
| ISSN: | 1846-7482 1330-0962 |
| Popis: | WOS: 000404505700013 PubMed: 28694726 Introduction: A nationwide multicentre study was conducted to establish well-defined reference intervals (Rls) of haematological parameters for the Turkish population in consideration of sources of variation in reference values (RVs). Materials and methods: K2-EDTA whole blood samples (total of 3363) were collected from 12 laboratories. Sera were also collected for measurements of iron, UIBC, TIBC, and ferritin for use in the latent abnormal values exclusion (LAVE) method. The blood samples were analysed within 2 hours in each laboratory using Cell Dyn and Ruby (Abbott), LH780 (Beckman Coulter), or XT-2000i (Sysmex). A panel of freshly prepared blood from 40 healthy volunteers was measured in common to assess any analyser-dependent bias in the measurements. The SD ratio (SDR) based on ANOVA was used to judge the need for partitioning RVs. Rls were computed by the parametric method with/without applying the LAVE method. Results: Analyser-dependent bias was found for basophils (Bas), MCHC, RDW and MPV from the panel test results and thus those Rls were derived for each manufacturer. Rls were determined from all volunteers' results for WBC, neutrophils, lymphocytes, monocytes, eosinophils, MCV, MCH and platelets. Gender-specific Rls were required for RBC, haemoglobin, haematocrit, iron, UIBC and ferritin. Region-specific Rls were required for RBC, haemoglobin, haematocrit, UIBC, and TIBC. Conclusions: With the novel use of a freshly prepared blood panel, manufacturer-specific Rls' were derived for Bas, Bas%, MCHC, RDW and MPV. Regional differences in Rls were observed among the 7 regions of Turkey, which may be attributed to nutritional or environmental factors, including altitude. Research Fund of Uludag Universty [UAP(T)-2011/48]; Abbott Diagnostics (Abbott Laboratories, IL, USA); Becton Dickinson (BD Diagnostics, Oxford, England) This study was supported by the Research Fund of Uludag Universty (UAP(T)-2011/48), Abbott Diagnostics (Abbott Laboratories, IL, USA) and Becton Dickinson (BD Diagnostics, Oxford, England). We wish to thank Dr. David Armbruster from Abbott Diagnostics (Abbott Laboratories, IL, USA) for his kind support for realization of the study. We especially thank all the volunteers for their participation in this study. Finally, we are grateful to Professor Andrew Myron Johnson for his detailed scientific editing of this manuscript. |
| Databáze: | OpenAIRE |
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