Ability of human CDC25B phosphatase splice variants to replace the function of the fission yeast Cdc25 cell cycle regulator
Autor: | Bernard Ducommun, Odile Mondesert, Béatrix Bugler, Matthieu Lemaire |
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Přispěvatelé: | Laboratoire de Biologie Cellulaire et Moléculaire du Contrôle de la Prolifération (LBCMCP), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre de Biologie Intégrative (CBI), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) |
Jazyk: | angličtina |
Rok vydání: | 2004 |
Předmět: |
Cell cycle checkpoint
Cell Cycle Proteins MESH: Cell Cycle MESH: DNA Replication Applied Microbiology and Biotechnology MESH: Recombinant Proteins chemistry.chemical_compound 0302 clinical medicine MESH: cdc25 Phosphatases 0303 health sciences MESH: Alternative Splicing Cell Cycle General Medicine Cell cycle MESH: ras-GRF1 Recombinant Proteins 3. Good health Cell biology DNA replication checkpoint Isoenzymes MESH: Schizosaccharomyces Biochemistry 030220 oncology & carcinogenesis RNA splicing MESH: Isoenzymes MESH: Fungal Proteins biological phenomena cell phenomena and immunity DNA Replication Cdc25 Ultraviolet Rays Genes Fungal [SDV.BC]Life Sciences [q-bio]/Cellular Biology Biology Microbiology Fungal Proteins 03 medical and health sciences MESH: Cell Cycle Proteins Schizosaccharomyces Humans cdc25 Phosphatases 030304 developmental biology MESH: DNA Damage MESH: Humans ras-GRF1 MESH: Gamma Rays biology.organism_classification Yeast Genes cdc Alternative Splicing chemistry MESH: Genes cdc Gamma Rays Schizosaccharomyces pombe biology.protein MESH: Ultraviolet Rays MESH: Genes Fungal DNA DNA Damage |
Zdroj: | FEMS Yeast Research FEMS Yeast Research, Oxford University Press (OUP), 2004, 5 (3), pp.205-11. ⟨10.1016/j.femsyr.2004.07.003⟩ |
ISSN: | 1567-1356 1567-1364 |
DOI: | 10.1016/j.femsyr.2004.07.003⟩ |
Popis: | CDC25 phosphatases are essential and evolutionary-conserved actors of the eukaryotic cell cycle control. To examine and compare the properties of three splicing variants of human CDC25B, recombinant fission yeast strains expressing the human proteins in place of the endogenous Cdc25 were generated and characterized. We report, that the three CDC25B variants: (i) efficiently replace the yeast counterpart in vegetative growth, (ii) partly restore the gamma and UV radiation DNA damage-activated checkpoint, (iii) fail to restore the DNA replication checkpoint activated by hydroxyurea. Although these yeast strains do not reveal the specific functions of the human CDC25B variants, they should provide useful screening tools for the identification of new cell cycle regulators and pharmacological inhibitors of CDC25 phosphatase. |
Databáze: | OpenAIRE |
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