Ability of human CDC25B phosphatase splice variants to replace the function of the fission yeast Cdc25 cell cycle regulator

Autor: Bernard Ducommun, Odile Mondesert, Béatrix Bugler, Matthieu Lemaire
Přispěvatelé: Laboratoire de Biologie Cellulaire et Moléculaire du Contrôle de la Prolifération (LBCMCP), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre de Biologie Intégrative (CBI), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2004
Předmět:
Cell cycle checkpoint
Cell Cycle Proteins
MESH: Cell Cycle
MESH: DNA Replication
Applied Microbiology and Biotechnology
MESH: Recombinant Proteins
chemistry.chemical_compound
0302 clinical medicine
MESH: cdc25 Phosphatases
0303 health sciences
MESH: Alternative Splicing
Cell Cycle
General Medicine
Cell cycle
MESH: ras-GRF1
Recombinant Proteins
3. Good health
Cell biology
DNA replication checkpoint
Isoenzymes
MESH: Schizosaccharomyces
Biochemistry
030220 oncology & carcinogenesis
RNA splicing
MESH: Isoenzymes
MESH: Fungal Proteins
biological phenomena
cell phenomena
and immunity

DNA Replication
Cdc25
Ultraviolet Rays
Genes
Fungal

[SDV.BC]Life Sciences [q-bio]/Cellular Biology
Biology
Microbiology
Fungal Proteins
03 medical and health sciences
MESH: Cell Cycle Proteins
Schizosaccharomyces
Humans
cdc25 Phosphatases
030304 developmental biology
MESH: DNA Damage
MESH: Humans
ras-GRF1
MESH: Gamma Rays
biology.organism_classification
Yeast
Genes
cdc

Alternative Splicing
chemistry
MESH: Genes
cdc

Gamma Rays
Schizosaccharomyces pombe
biology.protein
MESH: Ultraviolet Rays
MESH: Genes
Fungal

DNA
DNA Damage
Zdroj: FEMS Yeast Research
FEMS Yeast Research, Oxford University Press (OUP), 2004, 5 (3), pp.205-11. ⟨10.1016/j.femsyr.2004.07.003⟩
ISSN: 1567-1356
1567-1364
DOI: 10.1016/j.femsyr.2004.07.003⟩
Popis: CDC25 phosphatases are essential and evolutionary-conserved actors of the eukaryotic cell cycle control. To examine and compare the properties of three splicing variants of human CDC25B, recombinant fission yeast strains expressing the human proteins in place of the endogenous Cdc25 were generated and characterized. We report, that the three CDC25B variants: (i) efficiently replace the yeast counterpart in vegetative growth, (ii) partly restore the gamma and UV radiation DNA damage-activated checkpoint, (iii) fail to restore the DNA replication checkpoint activated by hydroxyurea. Although these yeast strains do not reveal the specific functions of the human CDC25B variants, they should provide useful screening tools for the identification of new cell cycle regulators and pharmacological inhibitors of CDC25 phosphatase.
Databáze: OpenAIRE