X-Ray Microtomography Reveals the 3d Enzymatic Deconstruction Pathway of Raw Lignocellulosic Biomass
| Autor: | Blosse, S, Bouchoux, Antoine, Montanier, C, Duru, Paul |
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| Přispěvatelé: | Institut de mécanique des fluides de Toulouse (IMFT), Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT), Toulouse Biotechnology Institute (TBI), Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), internal grant from the French National Research Institute for Agriculture, Food and Environment (INRAE), project Splendid. |
| Rok vydání: | 2022 |
| Předmět: |
History
Environmental Engineering Polymers and Plastics Renewable Energy Sustainability and the Environment Bioengineering Wheat straw [CHIM.MATE]Chemical Sciences/Material chemistry Industrial and Manufacturing Engineering Enzymatic hydrolysis X-ray microtomography Plant cell wall Business and International Management 3D imaging processing Waste Management and Disposal |
| Zdroj: | Bioresource Technology Reports Bioresource Technology Reports, 2023, 21, pp.101351. ⟨10.1016/j.biteb.2023.101351⟩ |
| ISSN: | 1556-5068 2589-014X |
| Popis: | International audience; In the context of the development of a circular bioeconomy, plant biomass constitutes a vast potential source of renewable molecules of interest. The industrial process essentially consists in using enzymes for its deconstruction. However, we are still lacking a precise understanding of the enzymes' action on such a complex, multiscale porous material. Here, we demonstrate that X-ray microtomography tackles this question in a unique way, making it possible to follow precisely the enzymatic degradation of a model biomass, wheat straw. We perform a thorough analysis of the imaging results over the full length scale of the sample and as a function of degradation time. In particular, we observe the progressive disappearance of specific cellulose-rich cell walls, with distinct 3D heterogeneities in the enzymatic action at different times and length scales. Those 3D pieces of information about the degradation process contrast clearly with those obtained from classical 2D imaging techniques. |
| Databáze: | OpenAIRE |
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