Overexpression of HPV16 E6/E7 mediated HIF-1α upregulation of GLUT1 expression in lung cancer cells

Autor: Rong Fan, Shuli Liu, Guang-Ping Wu, Yu-Jie Zhao, Wei-Jian Hou, Enhua Wang, Xueshan Qiu
Rok vydání: 2015
Předmět:
0301 basic medicine
Adult
Gene Expression Regulation
Viral
Male
Transcriptional Activation
Pathology
medicine.medical_specialty
Lung Neoplasms
Papillomavirus E7 Proteins
Immunocytochemistry
Cell
Gene Expression
Adenocarcinoma
03 medical and health sciences
0302 clinical medicine
Downregulation and upregulation
Cell Line
Tumor
medicine
Carcinoma
Humans
Lung cancer
Aged
Glucose Transporter Type 1
Human papillomavirus 16
Lung
business.industry
Papillomavirus Infections
Cancer
General Medicine
Oncogene Proteins
Viral
Middle Aged
medicine.disease
Hypoxia-Inducible Factor 1
alpha Subunit
Warburg effect
respiratory tract diseases
Up-Regulation
Gene Expression Regulation
Neoplastic
Repressor Proteins
030104 developmental biology
medicine.anatomical_structure
030220 oncology & carcinogenesis
Case-Control Studies
Female
business
Zdroj: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. 37(4)
ISSN: 1423-0380
Popis: High-risk human papillomavirus (HPV) infection may play an important role in non-small cell lung carcinoma (NSCLC) development. However, some recent studies have proved that it was not directly associated with lung cancer. The aim of this study was to evaluate the underlying molecular mechanism that HPV16 regulate the expression of GLUT1 and may promote the development of lung cancer. HPV16, HIF-1α, and GLUT1 were detected in pleural effusions of patients with lung cancer (n = 95) and with benign lung disease (n = 55) by immunocytochemistry. Western blotting and qRT-PCR were used to detect the expression chances of HPV16 E6/E7, HIF-1α, and GLUT1 in lung cancer cells. HPV16, HIF-1α, and GLUT1 were significantly more likely to be expressed in the malignant group than in the benign group as detected by immunocytochemistry (ICC), and HIF-1α was significantly correlated with HPV16 or GLUT1 in the malignant group (P
Databáze: OpenAIRE
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