Anti-Mouse Mesangial Cell Serum Induces Acute Glomerulonephropathy in Mice
| Autor: | Michael C. Braun, Jennie W. Owens, Yoshikage Yo, Hideko Mobaraki, Huiyan Lu, Jeffrey B. Kopp, Shashi Shrivastav, Laura Barisoni |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
medicine.medical_specialty
Time Factors Physiology Dose-Response Relationship Immunologic Immunoglobulins Apoptosis Mice Inbred Strains Mice SCID Biology Kidney Glomerulonephritis Membranous Cell Line Mice Mice Congenic chemistry.chemical_compound Antibody Specificity Glomerulopathy Lactate dehydrogenase Internal medicine Genetics medicine Animals Lung Mice Inbred BALB C Sheep Mesangial cell Cell growth Immune Sera 3T3 Cells General Medicine medicine.disease Glomerular Mesangium Endotoxins Disease Models Animal Endocrinology chemistry Mesangiolysis Organ Specificity Nephrology Acute Disease Antigens Surface Female Tuft cell Nephritis Cell Division |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1660-2129 |
| Popis: | In order to develop a model in mouse similar to anti- Thy-1 nephritis in the rat, we prepared sheep antiserum against SV40-transformed mouse mesangial (MES 13) cells. In vivo, the anti-mouse mesangial cell serum-treated mice showed severe azotemia that peaked at day 6 and proteinuria that peaked at day 8, in a dose-dependent fashion. Light microscopy and electron microscopy showed duplication of glomerular basement membranes, mesangiolysis, subendothelial and mesangial electron-dense deposits, and foot process effacement. Intraglomerular tuft cell number was significantly reduced at day 4 and there were increased numbers of apoptotic cells at days 2 and 4. SCID mice and mice lacking C3 manifested similar responses to anti-mouse mesangial cell serum, suggesting that T cells, B cells and complement are not required for glomerular injury in this model. In vitro, anti-mouse mesangial cell serum treated mesangial cells showed greater release of lactate dehydrogenase, decreased cell survival, and increased apoptotic cell death. Anti-mouse mesangial cell serum induces glomerulopathy characterized by mesangiolysis and mesangial cell apoptosis, and followed by cellular proliferation. |
| Databáze: | OpenAIRE |
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