The primary structure of UK 114 tumor antigen
| Autor: | Ludovica Faotto, Armando Negri, Severino Ronchi, Alberto Bartorelli, Bruno Berra, Irma Colombo, Fabrizio Ceciliani |
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| Jazyk: | angličtina |
| Předmět: |
UK 114
Molecular Sequence Data Biophysics Biochemistry Amino acid sequence Antigen Structural Biology Antigens Neoplasm Heat shock protein Genetics Animals Humans Cyanogen Bromide Molecular Biology Peptide sequence Autoanalysis Molecular mass Edman degradation Sequence Homology Amino Acid Chemistry Goats Protein primary structure Metalloendopeptidases Cell Biology Molecular biology Tumor antigen Peptide Fragments Neoplasm Proteins Rats Liver Acetylation Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization |
| Zdroj: | FEBS Letters. (2-3):147-150 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/0014-5793(96)00850-2 |
| Popis: | UK114 is a tumor antigen expressed by various malignant neoplasms. The complete amino acid sequence of UK114 purified from goat liver has been determined by automated Edman degradation of CNBr and endoproteinase Lys-C peptides. The protein contains 137 amino acid residues, which corresponds to a molecular mass of 14 229 Da. MALDITOF analysis resulted in a molecular weight of 14 290, suggesting that the N-terminal Met residue is acetylated. Sequence comparison shows that UK114 from goat liver (1) has 77% identity with a previously described 23 kDa protein from rat liver (Levy-Favatier et al. (1993) Eur. J. Biochem. 212, 665–673), (2) shares a very high degree of similarity with a family of prokaryotic and eukaryotic hypothetic proteins whose function have not yet been characterized, and (3) exhibits a significant similarity to a group of tumor-associated antigens which belongs to a superfamily of heat shock proteins, acting as possible targets for the host's antitumor immunity. |
| Databáze: | OpenAIRE |
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