The Drosophila mobile element jockey belongs to LINEs and contains coding sequences homologous to some retroviral proteins
| Autor: | Lev J. Mizrokhi, Andres F. Priimägi, Y. V. Ilyin |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Transposable element
Molecular Sequence Data Restriction Mapping Homology (biology) Genetics Animals Amino Acid Sequence ORFS Genomic organization biology Base Sequence Nucleic acid sequence RNA-Binding Proteins RNA-Directed DNA Polymerase General Medicine DNA biology.organism_classification Long interspersed nuclear element Open reading frame Drosophila melanogaster Retroviridae DNA Transposable Elements RNA Polymerase II Carrier Proteins |
| Zdroj: | Gene. 70(2) |
| ISSN: | 0378-1119 |
| Popis: | A detailed investigation of the Drosophila melanogaster mobile dispersed repetitive element jockey was performed. This is similar in its structural organization and coding potential to the long interspersed elements (LINEs) of various organisms. A complete copy of jockey (approx. 5 kb) is terminated with an oligodeoxynu-cleotide (dA) sequence preceded by two long open reading frames (ORFs) overlapping with a frameshift-1. Judging by the sequence homologies, ORF1 codes for a nucleic-acid-binding protein, and ORF2 for a reverse transcriptase which is most similar in its sequence to putative reverse transcriptase of other LINEs. As demonstrated by sequencing two deleted jockey copies, they contain only a small part of ORF2; however, other regions, including the terminal sequences, are highly conservative. The existence of a large number of jockey copies with a deletion in the second frame may indicate that they can use reverse transcriptase in trans . |
| Databáze: | OpenAIRE |
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