Polymerizing equilibria in neurophysin
| Autor: | M. Ginsburg, G. D. Burford, P. J. Thomas |
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| Rok vydání: | 1969 |
| Předmět: |
Chromatography
Aqueous solution Polymers Swine Vasopressins Physiology Chemistry Elution Lysine Dispersity Derivative analysis Articles Neurophysins Ion Molecular Weight Solutions Polymerization Chromatography Gel Animals Calcium Magnesium Pituitary Hormones Posterior hormones hormone substitutes and hormone antagonists Protein Binding Lysine vasopressin |
| Zdroj: | The Journal of Physiology. 205:635-646 |
| ISSN: | 0022-3751 |
| Popis: | 1. Evidence for the occurrence of a rapidly polymerizing equilibrium system in freshly prepared aqueous solutions of porcine neurophysin has been obtained by frontal and zonal analysis of gel-filtration elution patterns. 2. Elution volumes increased with decreasing protein concentration and derivative analysis of fronts suggested the existence of different polymerizing forms varying in relative proportion with protein concentration. 3. In the presence of lysine vasopressin, the results suggested a shift of the equilibrium in favour of higher polymerizing forms. 4. In the presence of Ca2+ elution patterns became characteristic of a monodisperse system of relatively high molecular weight. The effect of Ca2+ did not survive removal of the ion and was not reproduced by Mg2+. 5. Evidence for a slowly developing polymerization of neurophysin in aqueous solution has been obtained in experiments with solution prepared 24 hr before subjection to gel-filtration. 6. A model is proposed for the description of the binding of lysine vasopressin to porcine neurophysin. |
| Databáze: | OpenAIRE |
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