EFEITO DA SUPLEMENTAÇÃO COM PROBIÓTICOS EM ANASTOMOSES COLÔNICAS DE RATOS: ESTUDO MORFOLÓGICO E TENSIOMÉTRICO
| Autor: | Eneri Vieira de Souza Leite Mello, Tiago Jacometo Coelho de Castilho, Gustavo Henrique Doná Rodrigues de Almeida, Antonio Carlos Ligocki Campos |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
medicine.medical_specialty Lactobacillus paracasei RD1-811 Collagen densitometry Wound healing Tensiômetro RC799-869 Anastomosis Placebo Probiotic Colonic anastomosis Gastroenterology law.invention 03 medical and health sciences 0302 clinical medicine Lactobacillus acidophilus Lactobacillus rhamnosus Fibrosis law Cicatrização ferida Densitometria do colágeno Internal medicine medicine Tensiometers Animals Rats Wistar 030304 developmental biology Ratos 0303 health sciences biology Anastomose colônica business.industry Lacticaseibacillus rhamnosus Probiotics Histology General Medicine Diseases of the digestive system. Gastroenterology medicine.disease biology.organism_classification Rats Intestines 030211 gastroenterology & hepatology Original Article Probiótico Surgery business |
| Zdroj: | ABCD: Arquivos Brasileiros de Cirurgia Digestiva, Vol 33, Iss 4 (2021) ABCD. Arquivos Brasileiros de Cirurgia Digestiva (São Paulo), Volume: 33, Issue: 4, Article number: e1550, Published: 25 JAN 2021 Arquivos Brasileiros de Cirurgia Digestiva : ABCD ABCD. Arquivos Brasileiros de Cirurgia Digestiva (São Paulo) v.33 n.4 2020 ABCD. Arquivos Brasileiros de Cirurgia Digestiva (São Paulo) Colégio Brasileiro de Cirurgia Digestiva (CBCD) instacron:CBCD |
| ISSN: | 0102-6720 |
| Popis: | Background: The use of probiotics positively modifies the composition and function of intestinal flora, improving the quality of intestinal anastomosis. Aim: To evaluate the impact of probiotic use on intestinal anastomosis of rats. Method: Thirty-six adult male Wistar rats (Rattus norvegicus albinus, Rodentia Mammalia) were used, with body weight ranging from 220-320 g. The animals were housed and acclimated individually in boxes receiving water and ration ad libitum. After initial acclimatization, the control group received perioperative ration ad libitum for 12 days (seven preoperatively and five postoperatively) associated with the maltodextrin formula at a dose of 250 mg/day in isocaloric and isovolumetric form. Likewise, the probiotic group received oral supplementation of probiotics dose of 250 mg/day, associated with isocaloric and isovolumetric diet. The probiotic chosen for this study was composed of strains (doses 1x109 CFU/g)12 Lactobacillus paracasei LPC-37, Bifidobacterium lactis HN0019, Lactobacillus rhamnosus HN001 and Lactobacillus acidophilus NCFM. Probiotics or placebo were administered orally with the aid of a dosimeter spatula. Both groups underwent two colostomies, one in the right colon and the second in rectosigmoid, followed by reanastomosis with eight separate 6-0 mononylon stitches. The sacrifice took place on the fifth day. The parameters evaluated included tensile strength, histology and collagen densitometry. Results: The rate of intestinal fistula for the control and probiotic groups were, respectively, 22.22% and 11.11% (p=0.6581).Perioperative supplementation with probiotics increased collagen deposition of types I and III (p |
| Databáze: | OpenAIRE |
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