Succinylated gelatine: an alternative to hydroxyethyl starch for labelling leukocytes with 99mTc-HMPAO
| Autor: | M. Roca, Félix Armero, Ignacio R. Jimenez, Josep Martin-Comin, Carmen Díaz, A. Rodríguez-Gasén |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
medicine.medical_specialty
Hydroxyethyl starch Scintigraphy Hydroxyethyl Starch Derivatives Leukocyte Count chemistry.chemical_compound Technetium Tc 99m Exametazime White blood cell Labelling Leukocytes medicine Humans Radiology Nuclear Medicine and imaging Platelet Radionuclide Imaging Eosin Y Cells Cultured Chromatography Red Cell medicine.diagnostic_test Succinates General Medicine In vitro Surgery medicine.anatomical_structure chemistry Isotope Labeling Gelatin Radiopharmaceuticals medicine.drug |
| Zdroj: | Nuclear Medicine Communications. 26:749-752 |
| ISSN: | 0143-3636 |
| DOI: | 10.1097/01.mnm.0000172741.36488.f7 |
| Popis: | Background Hydroxyethyl starch (HES) is the most used plasma expander in the sedimentation of the erythrocytes during the radiolabelling procedure for leukocytes in vitro. Aim To evaluate the usefulness of succinylated gelatine (GEL), another colloidal plasma expander, as an alternative to HES in this process. Methods Two identical blood samples were obtained from 30 patients referred to white blood cell scintigraphy. The first sample was used to label leukocytes with 9 9 m Tc-HMPAO using the routine procedure, with HES. The other sample was used to label leukocytes with 9 9 m Tc.HMPAO using the same procedure, with GEL. The cell concentration of the leukocyte-platelet-rich plasma (LPRP) achieved after blood sedimentation was analysed. Labelling efficiency was calculated and the eosin Y viability and red cell/ leukocyte ratio were evaluated from the final labelled cell suspension. Results Leukocytes and platelets recovered in LPRP were not statistically different between both HES and GEL samples (leukocytes: 8.10 x 10 3 /μl ′ 3.82 and 7.80 x 103/ μl ′ 3.47; platelets: 411x1 10 3 /μl ′ 182 and 406 x 10 3 / μl ′ 172, respectively). There were no significant differences between both agents on the labelling efficiency (HES: 80.3% ′ 6.6%; GEL: 80.1 % ′ 6.3%), the eosin Y viability (HES: 99.2% ′ 1.3%; GEL: 99.3% ′ 1.1%) and the red cell/leukocyte ratio (HES: 1.21 ′ 0.7; GEL: 0.9 ′ 0.5). Conclusion These results show that succinylated gelatine can be used instead of hydroxyethyl starch in the labelling of leukocytes with 9 9 m Tc-HMPAO. |
| Databáze: | OpenAIRE |
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