Purification and partial characterization of fimbriae of Vibrio cholerae O1
| Autor: | Masaaki Iwanaga, Shoichi Shimotori, Masahiko Ehara, Mikako Ishibashi, Tatsuro Naito, Yoshio Ichinose |
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| Rok vydání: | 1987 |
| Předmět: |
Serotype
animal structures Hot Temperature Hemagglutination Fimbria Neuraminidase Biology medicine.disease_cause El Tor Microbiology Agar plate Epitopes medicine Humans Centrifugation Vibrio cholerae Antigens Bacterial General Veterinary General Immunology and Microbiology Public Health Environmental and Occupational Health biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification female genital diseases and pregnancy complications Microscopy Electron Infectious Diseases Fimbriae Bacterial bacteria Molecular Medicine Homogenization (biology) |
| Zdroj: | Vaccine. 5(4) |
| ISSN: | 0264-410X |
| Popis: | Fimbriae of Vibrio cholerae O1 were purified from a strain of the classical biotype, Inaba serotype (Bgd 17), and a strain of the El Tor biotype, Ogawa serotype (K23), grown on TCG agar medium by the following procedure; homogenization of the cell suspension to detach fimbriae, ultracentrifugation to remove remaining cells and their debris, concentration of the supernatant containing fimbriae with ultrafiltration, and 20 to 40% sucrose linear gradient centrifugation of the concentrated material. The fimbriae in both preparations were flexible, long fibres readily distinguishable under the electron microscope from those of CFA/I, CFA/II seen in ETEC strains. Their structural subunit was a protein of 16 kdaltons. Fimbriae isolated from both serotypes and biotypes shared antigenic determinants. |
| Databáze: | OpenAIRE |
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