Comparative study of colorimetric DNA hybridization method and conventional culture procedure for detection of Salmonella in foods
| Autor: | Kevan Whippie, Samuel W. Chan, Anna Whilby, Stephen G. Wilson, Alpa Shah, Andrew Johnson, Mark A Mozola, Donald Neil Halbert, Marcela Vera-Garcia, Margaret Ottaviani |
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| Rok vydání: | 1990 |
| Předmět: |
DNA
Bacterial Salmonella Chromatography Oligonucleotide DNA–DNA hybridization Culture Procedure Nucleic Acid Hybridization Food Contamination General Chemistry Dipstick Biology medicine.disease_cause biology.organism_classification Enterobacteriaceae Microbiology Nucleic acid thermodynamics medicine Food Microbiology Animals Colorimetry Bacteria |
| Zdroj: | Journal - Association of Official Analytical Chemists. 73(3) |
| ISSN: | 0004-5756 |
| Popis: | A second generation nucleic acid hybridization assay has been developed and evaluated against the conventional culture method for detection of salmonellae In foods. The assay Involves a liquid hybridization with Sa/mone/fo-speclflc oligonucleotide probes, capture of probe:target hybrids onto a solid support (plastic dipstick), and a colorimetric end point detection. The assay can be completed In 2.5 h, following approximately 44 h of culture enrichment. One thousand samples representing 20 food types were analyzed in parallel by both methods. Samples Included unlnoculated test product, and product Inoculated with Salmonella at 2 levels. Eighteen Salmonella serotypes were used as Inocula. The data demonstrate that the colorlmetric hybridization method and the conventional culture method are equivalent In their ability to detect Salmonella contamination of foods. |
| Databáze: | OpenAIRE |
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