General and hybrid correlation nuclear magnetic resonance analysis of phosphorus in Phytophthora palmivora
| Autor: | Michael J. Adams, Gemma M. Kirwan, Julie O. Niere, David I. Fernandez |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Phytophthora
chemistry.chemical_classification Magnetic Resonance Spectroscopy Sugar phosphates biology Polyphosphate Phosphorus Phytophthora palmivora Biophysics chemistry.chemical_element Cell Biology Cleavage (embryo) biology.organism_classification Biochemistry Phosphonate chemistry.chemical_compound Chain length Nuclear magnetic resonance chemistry Multivariate Analysis Correlation analysis sense organs Molecular Biology |
| Zdroj: | Analytical Biochemistry. 429:1-7 |
| ISSN: | 0003-2697 |
| Popis: | Generalized two-dimensional (Gen2D) correlation analysis and hybrid correlation analysis have been applied to a series of dynamic (31)P nuclear magnetic resonance (NMR) spectra to monitor the in vivo metabolic changes of the plant pathogen Phytophthora palmivora in the presence and absence of phosphonate over an 18-h period. Results indicate that phosphonate exposure causes cleavage in organism polyphosphate chains as well as an increase in total sugar phosphates. In the presence of phosphonate, the NMR resonances attributed to terminal polyphosphate phosphorus reduced at a lower rate than those of middle polyphosphate phosphorus, indicating a change in average chain length and suggesting cleavage in the middle of the chain as well as at the ends. The correlation analysis techniques serve to identify and confirm spectral regions undergoing major change in the time-series data and facilitate the analysis of these dynamic changes. |
| Databáze: | OpenAIRE |
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