Pouvoir immunomodulateur d'une souche de Mycobacterium chelonae 1. Réponse, in vitro, des lymphocytes murins
| Autor: | Charles Pilet, Jean-Marc Person, Francine Barrat, Tsehay Neway, Henri-Jean Boulouis |
|---|---|
| Přispěvatelé: | Laboratoire des autres chaires du département de pathologie animale, Institut National de la Recherche Agronomique (INRA) |
| Rok vydání: | 1989 |
| Předmět: |
Lymphocyte
Immunology Mycobacterium chelonae Lymphocyte Activation Microbiology Mycobacterium Mice chemistry.chemical_compound Splenocyte medicine Animals Immunology and Allergy Mice Inbred BALB C Mice Inbred C3H General Veterinary biology General Medicine biology.organism_classification Molecular biology In vitro Mice Inbred C57BL Infectious Diseases medicine.anatomical_structure chemistry Mice Inbred DBA Concanavalin A Polyclonal antibodies biology.protein [SDV.IMM]Life Sciences [q-bio]/Immunology Female Thymidine Spleen |
| Zdroj: | Comparative Immunology, Microbiology and Infectious Diseases Comparative Immunology, Microbiology and Infectious Diseases, Elsevier, 1989, 12 (3), pp.63-70 |
| ISSN: | 0147-9571 |
| DOI: | 10.1016/0147-9571(89)90050-7 |
| Popis: | Immunomodulatory properties of a strain of live Mycobacterium chelonae (Mch) was investigated in an in vitro lymphocyte transformation system. Murine splenocyte activation by this bacterium was characterized by polyclonal lymphoproliferative responses in a dose dependent fashion. Optimal doses ranging from 20 to 80 micrograms of Mch (wet weight) per ml of cell suspension induced a very significant mitogenic effect. Higher doses (100 micrograms) of Mch manifested a decreased rate of tritiated thymidine ([3H] TdR) uptake whereas responsiveness of splenic lymphocytes to lower doses (0.156 microgram) was not modified. Contrary to the splenocyte responses activation of murine thymocytes by this mycobacterium is characterised by a decreased proliferation as compared to the background count of unstimulated cells. Simultaneous addition of Mch with optimal doses of Concanavalin A (Con A) and Phytohemaglutinin (PHA) potentiated polyclonal mitogenic responses of murine splenocytes to these two lectins. However, proliferation of these lymphocytes to Lypopolysaccharide (LPS) induction was not modified. BALB/C and DBA/2 spenocytes were found to be more responsive to stimulation by this Mycobacterium as compared to those of C3H/Ou and to a lesser degree to those of C57BL/6 mice. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|