Dynamic characterization of drug resistance and heterogeneity of the gastric cancer cell BGC823 using single-cell Raman spectroscopy
| Autor: | Yuezhou Yu, Anpei Ye, Lin Shen, Ludi Jin, Jing Gao, Yong Zhang, Jingjing Xu |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Paclitaxel Cell Analytical chemistry Drug resistance Spectrum Analysis Raman 01 natural sciences Biochemistry Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound symbols.namesake Stomach Neoplasms Cell Line Tumor Electrochemistry medicine Environmental Chemistry Neoplasm Humans Spectroscopy 010401 analytical chemistry medicine.disease Molecular biology 0104 chemical sciences Intensity (physics) 030104 developmental biology medicine.anatomical_structure chemistry Cell culture Drug Resistance Neoplasm Cancer cell symbols Raman spectroscopy |
| Zdroj: | The Analyst. 143(1) |
| ISSN: | 1364-5528 |
| Popis: | Drug resistance and heterogeneous characteristics of human gastric carcinoma cells (BGC823) under the treatment of paclitaxel (PTX) were investigated using single-cell Raman spectroscopy (RS). RS of normal and drug-resistant BGC823 cells (DR-BGC823) were collected and analyzed using arithmetic, statistic and individual spectrum analysis. The dynamic effects of paclitaxel (PTX) in normal and DR-BGC823 cells were evaluated dynamically. The RS intensity changed with PTX over time and produced distinct different results for the two types of cells. The average RS intensities of the normal BGC823 cells initially decreased and then increased under PTX treatment after 24 hours. In contrast, upon exposure to PTX, the average intensity of the DR-BGC823 cells initially increased within 12 hours and then gradually decreased and approached a steady state. The temporal variation of the typical component in the cells was analyzed by comparing the ratios between Raman bands. More importantly, the heterogeneous characteristics of the BGC823 cells under PTX treatment were quantified and clustered using hierarchical trees combined with RS intensity changes. The 'outlier' cells related to drug resistance were discriminated. The heterogeneity of the normal BGC823 cells under drug treatment gradually appeared over time, and was evaluated with the eigenvalues of principal component analysis (PCA). Our study indicates that single-cell RS may be useful in systematically and dynamically characterizing the drug response of cancer cells at the single-cell level. |
| Databáze: | OpenAIRE |
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