Antibody Responses to Recombinant Epstein-Barr Virus Antigens in Nasopharyngeal Carcinoma Patients: Complementary Test of ZEBRA Protein and Early Antigens p54 and p138
| Autor: | A. Benslimane, Meriem Khyatti, D. Lang, Abdellatif Benider, R'kia Dardari, W. Hinderer, B. El Gueddari, Irene Joab |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Adult
Microbiology (medical) Herpesvirus 4 Human Adolescent Population Nasopharyngeal neoplasm Enzyme-Linked Immunosorbent Assay Biology Antibodies Viral medicine.disease_cause Sensitivity and Specificity Immunoglobulin G Serology Viral Proteins Antigen Virology otorhinolaryngologic diseases medicine Humans Child education Antigens Viral education.field_of_study Nasopharyngeal Neoplasms Middle Aged medicine.disease Epstein–Barr virus Recombinant Proteins Immunoglobulin A DNA-Binding Proteins stomatognathic diseases Nasopharyngeal carcinoma Immunology Trans-Activators biology.protein Antibody |
| Zdroj: | Journal of Clinical Microbiology. 39:3164-3170 |
| ISSN: | 1098-660X 0095-1137 |
| Popis: | Serological tests based on the antibodies directed against the Epstein-Barr virus early antigen (EA) and viral capsid antigen (VCA), which have been recognized as tumor markers for nasopharyngeal carcinoma (NPC), are routinely used to help in the diagnosis of this malignancy. The detection of these antibodies reveals very low titers, found only in a small proportion of young compared with older NPC patients. This is a problem for the diagnosis of NPC, especially among Maghrebians, among whom young people are also affected, and emphasizes the necessity to search for more reliable markers. The present study reports results of immunoglobulin G (IgG) and IgA responses of NPC patients to recombinant EA antigens p54 (BMRF1) and p138 (BALF2), VCA complex antigens p18 (BFRF3) and p23 (BLRF2), and EBNA antigen p72 (BKRF1). Our results show that IgA-EA-p54 and -p138 (IgA-EA-p54+138) antibodies have a diagnostic value for detection of NPC (70%), compared with IgA-VCA-p18+23 and IgA-EBNA-p72, which have limited diagnostic value, especially in young patients. It is also noteworthy that IgA-EA-p54+138 can detect a high percentage (64%) of NPC cases negative by immunofluorescence. These results, however, clearly show that a single test cannot achieve the objective of detecting all NPC patients, and it seems advisable to combine different tests for the diagnosis of NPC. The combination of IgG-ZEBRA with IgA-EA-p54+138 improved the sensitivity of detection of NPC to 95% in the overall NPC population. The use of IgA-EA-p54+138 in combination with IgG-ZEBRA will facilitate detailed studies on the pattern of antibody response, which may result in the development of useful serological markers to guide the treatment of NPC. |
| Databáze: | OpenAIRE |
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