Differential effect of recombinant human and mouse interferons on replication of herpes simplex virus type 1 in mouse cells
| Autor: | Richard J. Whitley, S. Chatterjee, E. R. Kern, U. Wintergerst, J.D. Gangemi |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
viruses
medicine.medical_treatment Herpesvirus 1 Human Biology Virus Replication medicine.disease_cause Virus 3T3 cells Herpesviridae Mice Viral Proteins Interferon Virology medicine Animals Humans Nucleocapsid Pharmacology Interferon-alpha 3T3 Cells Molecular biology Recombinant Proteins In vitro Microscopy Electron Cytokine Herpes simplex virus medicine.anatomical_structure Cell culture Interferon Type I medicine.drug |
| Zdroj: | Antiviral Research. 32:27-34 |
| ISSN: | 0166-3542 |
| DOI: | 10.1016/0166-3542(95)00975-2 |
| Popis: | Pretreatment of murine (BALB/3T3) cells with either murine or recombinant hybrid human B/D interferon (IFN) blocked the release of infectious herpes simplex virus type 1 (HSV-1) from treated cells. The block in replication was not due to an effect on attachment of HSV-1 to the target cells or to toxic effects of IFN. Immunoblot analyses showed that murine IFN significantly reduced the expression of virus-specific proteins in IFN-treated cells. In contrast, B/D IFN had no major effect on the expression of viral proteins in treated cells. In support of the above observation, electron microscopy of virus-infected cells displayed formation of nucleocapsids within the nucleus of IFN-treated cells. However, the expression of glycoproteins B and D was reduced in B/D IFN-treated cells. These results suggested that murine IFN blocked HSV-1 replication at an early stage whereas B/D IFN inhibited HSV-1 replication at a late stage in virus morphogenesis. |
| Databáze: | OpenAIRE |
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