Intravenous Anti-IL-5 Monoclonal Antibody Reduces Eosinophils and Tenascin Deposition in Allergen-Challenged Human Atopic Skin
| Autor: | Andrew Menzies-Gow, Simon Phipps, Yee Ean Ong, A.B. Kay, P. Flood-Page |
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| Rok vydání: | 2004 |
| Předmět: |
Adult
Allergy Pathology medicine.medical_specialty Adolescent Biopsy Tenascin Dermatology Antibodies Monoclonal Humanized Biochemistry Dermatitis Atopic Allergic inflammation Atopy 03 medical and health sciences 0302 clinical medicine anti-IL-5 medicine Humans eosinophil Molecular Biology Interleukin 5 Skin 030304 developmental biology 0303 health sciences biology business.industry Antibodies Monoclonal Cell Biology Allergens Middle Aged Eosinophil medicine.disease 3. Good health Eosinophils medicine.anatomical_structure late-phase reaction Injections Intravenous Immunology biology.protein Interleukin-5 Antibody business Mepolizumab 030215 immunology medicine.drug |
| Zdroj: | Journal of Investigative Dermatology. 122:1406-1412 |
| ISSN: | 0022-202X |
| Popis: | Anti-IL-5 monoclonal antibody (mepolizumab) reduces baseline bronchial mucosal eosinophils and deposition of extracellular matrix proteins in the reticular basement membrane in mild asthma. Here we report the effect of anti-IL-5, in the same patients, on allergen-induced eosinophil accumulation, tenascin deposition (as a marker of repair and remodelling) and the magnitude of the late-phase allergic cutaneous reaction. Skin biopsies were performed in 24 atopic subjects at allergen- and diluent-injected sites before 6 and 48 h after, three infusions of a humanized, monoclonal antibody against IL-5 (mepolizumab) using a randomized double-blind, placebo-controlled design. Anti-IL-5 significantly inhibited eosinophil infiltration in 6 h and 48 h skin biopsies as well as the numbers of tenascin immunoreactive cells at 48 h. In contrast, anti-IL-5 had no significant effect on the size of the 6 or 48 h late-phase cutaneous allergic reaction. This study (a) suggests that eosinophils are unlikely to cause the redness, swelling, and induration characteristic of the peak (6 h) late-phase cutaneous allergic reaction and (b) shows that decreases in tenascin positive cells at 48 h correlates with reduction of eosinophils, so providing further evidence of involvement in remodelling processes associated with allergic inflammation. |
| Databáze: | OpenAIRE |
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