Synthesis, characterization, and biological affinity of a near-infrared-emitting conjugated oligoelectrolyte
| Autor: | Carol A. Vandenberg, Guillermo C. Bazan, Jenny Du, Alexander W. Thomas, Zachary B. Henson |
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| Rok vydání: | 2014 |
| Předmět: |
Cell Survival
media_common.quotation_subject Endocytosis Biochemistry Catalysis Cercopithecus aethiops chemistry.chemical_compound Electrolytes Colloid and Surface Chemistry Yeasts Chlorocebus aethiops Escherichia coli Animals Propidium iodide Internalization Lipid bilayer Late endosome media_common Microscopy Microscopy Confocal Staining and Labeling Communication Optical Imaging Colocalization General Chemistry Staining Membrane chemistry Confocal COS Cells Chemical Sciences |
| Zdroj: | Journal of the American Chemical Society, vol 136, iss 10 Journal of the American Chemical Society |
| Popis: | A near-IR-emitting conjugated oligoelectrolyte (COE), ZCOE, was synthesized, and its photophysical features were characterized. The biological affinity of ZCOE is compared to that of an established lipid-membrane-intercalating COE, DSSN+, which has blue-shifted optical properties making it compatible for tracking preferential sites of accumulation. ZCOE exhibits diffuse staining of E. coli cells, whereas it displays internal staining of select yeast cells which also show propidium iodide staining, indicating ZCOE is a “dead” stain for this organism. Staining of mammalian cells reveals complete internalization of ZCOE through endocytosis, as supported by colocalization with LysoTracker and late endosome markers. In all cases DSSN+ persists in the outer membranes, most likely due to its chemical structure more closely resembling a lipid bilayer. |
| Databáze: | OpenAIRE |
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