RNA2-encoded VP37 protein of Broad bean wilt virus 1 is a determinant of pathogenicity, host susceptibility, and a suppressor of post-transcriptional gene silencing
| Autor: | Luis Rubio, Laura Elvira-González, Inmaculada Ferriol Safont, Ezio Peri, Salvatore Davino, C. Carpino, Vicente Medina, Luis Galipienso Torregrosa |
|---|---|
| Přispěvatelé: | Carpino C., Ferriol Safont I., Elvira-Gonzalez L., Medina V., Rubio L., Peri E., Davino S., Galipienso Torregrosa L. |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Agroinfiltration
Infectious clone Determinant of pathogenicity viruses determinant of pathogenicity Gene Expression Soil Science Nicotiana benthamiana Plant Science BBWV-1 determinant of pathogenicity Fabavirus infectious clone Secoviridae VSR BBWV‐1 Host Specificity BBWV-1 Virus Viral Proteins Broad bean wilt virus Solanum lycopersicum Tobacco H20 Plant diseases Molecular Biology Plant Diseases Virulence biology Secoviridae Turnip crinkle virus fungi infectious clone VSR food and beverages Settore AGR/12 - Patologia Vegetale RNA virus Original Articles Deteminant of Pathogenicity biology.organism_classification Potato virus X Virology Fabavirus Vicia faba RNA silencing Settore AGR/11 - Entomologia Generale E Applicata RNA Viral Original Article RNA Interference Capsicum Agronomy and Crop Science |
| Zdroj: | electronico ReDivia: Repositorio Digital del Instituto Valenciano de Investigaciones Agrarias Instituto Valenciano de Investigaciones Agrarias (IVIA) Repositorio Abierto de la UdL Universitad de Lleida Molecular Plant Pathology Recercat: Dipósit de la Recerca de Catalunya Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) ReDivia. Repositorio Digital del Instituto Valenciano de Investigaciones Agrarias Universidad de Alicante (UA) Recercat. Dipósit de la Recerca de Catalunya instname |
| Popis: | Broad bean wilt virus 1 (BBWV‐1, genus Fabavirus, family Secoviridae) is a bipartite, single‐stranded positive‐sense RNA virus infecting many horticultural and ornamental crops worldwide. RNA1 encodes proteins involved in viral replication whereas RNA2 encodes two coat proteins (the large and small coat proteins) and two putative movement proteins (MPs) of different sizes with overlapping C‐terminal regions. In this work, we determined the role played by the small putative BBWV‐1 MP (VP37) on virus pathogenicity, host specificity, and suppression of post‐transcriptional gene silencing (PTGS). We engineered a BBWV‐1 35S‐driven full‐length cDNA infectious clone corresponding to BBWV‐1 RNA1 and RNA2 (pBBWV1‐Wt) and generated a mutant knocking out VP37 (pBBWV1‐G492C). Agroinfiltration assays showed that pBBWV1‐Wt, as the original BBWV‐1 isolate, infected broad bean, tomato, pepper, and Nicotiana benthamiana, whereas pBBWV1‐G492C did not infect pepper and tomato systemically. Also, pBBWV1‐G492C induced milder symptoms in broad bean and N. benthamiana than pBBWV1‐Wt. Differential retrotranscription and amplification of the (+) and (−) strands showed that pBBWV1‐G492C replicated in the agroinfiltrated leaves of pepper but not in tomato. All this suggests that VP37 is a determinant of pathogenicity and host specificity. Transient expression of VP37 through a potato virus X (PVX) vector enhanced PVX symptoms and induced systemic necrosis associated with programmed cell death in N. benthamiana plants. Finally, VP37 was identified as a viral suppressor of RNA silencing by transient expression in N. benthamiana 16c plants and movement complementation of a viral construct based on turnip crinkle virus (pTCV‐GFP). In this work, we determined using different approaches the role played by the small putative broad bean wilt virus 1 MP (VP37) on virus pathogenicity, host specificity, and suppression of post‐transcriptional gene silencing. |
| Databáze: | OpenAIRE |
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