Resveratrol inhibits phosphorylation within the signal transduction and activator of transcription 3 signaling pathway by activating sirtuin 1 in SW1353 chondrosarcoma cells
| Autor: | Jun Pan, Kehe Yu, Haidong Jin, Ningyu Cai, Bin Li, Jingdong Zhang, Hui Chen |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
STAT3 Transcription Factor
0301 basic medicine Cancer Research animal structures Cell Survival Chondrosarcoma Apoptosis Bone Neoplasms Resveratrol Biochemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Bcl-2-associated X protein Sirtuin 1 Cell Line Tumor Stilbenes Genetics Humans Viability assay Phosphorylation Molecular Biology Cell Proliferation bcl-2-Associated X Protein Dose-Response Relationship Drug biology Caspase 3 Cell growth Transfection Cell cycle Antineoplastic Agents Phytogenic Molecular biology Cell biology 030104 developmental biology Proto-Oncogene Proteins c-bcl-2 Oncology chemistry Cell culture 030220 oncology & carcinogenesis biology.protein Molecular Medicine Signal transduction Signal Transduction |
| Zdroj: | Molecular Medicine Reports. 14:2685-2690 |
| ISSN: | 1791-3004 1791-2997 |
| Popis: | The present study assessed the mechanism by which resveratrol (Res) inhibits the growth of SW1353 chondrosarcoma cells and examined whether sirtuin 1 (Sirt1) activation affects phosphorylation within the signal transduction and activator of transcription 3 (STAT3) signaling pathway. The present study used SW1353 chondrosarcoma cells in the logarithmic phase of growth (control and treatment groups). The latter group was treated with Res at 25 and 50 µmol/l for 24 h, and cell viability, proliferation and apoptosis were analyzed using the cell counting kit‑8 assay, colony counting and Hoechst staining, respectively. The expression levels of caspase‑3, cleaved caspase‑3, B‑cell lymphoma‑2 (BCL‑2), BCL-2 associated X protein (Bax), STAT3 and phosphorylated (p‑)STAT3) were measured by Western blotting. SW1353 cells were transfected with small interfering (si)RNA targeting Sirt1 and the expression levels of Sirt1, STAT3 and p-STAT3 were assessed. Exposure of SW1353 cells to Res reduced cell viability in a dose‑dependent manner (P |
| Databáze: | OpenAIRE |
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