Production of interferon byTheileria annulata-andT. parva-infected bovine lymphoid cell lines
| Autor: | Eberhard Schein, R. O. Williams, S. Steuber, J. S. Ahmed, P. Wiegers, Dirk A. E. Dobbelaere |
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| Rok vydání: | 1993 |
| Předmět: |
Theileria parva
Gene Expression Cell Line Pathogenesis Interferon-gamma Interferon Complementary DNA parasitic diseases medicine Animals Interferon gamma Lymphocytes RNA Messenger Northern blot General Veterinary biology Lymphoblast General Medicine biology.organism_classification Theileria annulata Virology Infectious Diseases Cell culture Insect Science Cattle Parasitology medicine.drug |
| Zdroj: | Parasitology Research. 79:178-182 |
| ISSN: | 1432-1955 0044-3255 |
| Popis: | Theileria annulata and T. parva-infected lymphoblastoid cells were examined for their capacity to produce interferon (IFN). Supernatants of such cells were tested in biological assay for their antiviral activity. Only T. parva-infected cells of T-cell origin were capable of producing IFN-gamma. Supernatants of some but not all T. annulata-infected cells showed also antiviral activity, which was greatly reduced after exposure to a pH of 2. Northern-blot analysis of the cells using an IFN-gamma cDNA probe confirmed the results obtained for T. parva-infected cells in a biological assay. No IFN-gamma mRNA was detected in T. annulata-infected cells. The importance of IFN for the pathogenesis of theileriosis is discussed. |
| Databáze: | OpenAIRE |
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