FDG-PET/CT Enables the Detection of Recurrent Same-Site Deep Vein Thrombosis by Illuminating Recently Formed, Neutrophil-Rich Thrombus
| Autor: | Michael R. Jaff, Jessica Truelove, Megan H. MacNabb, Tetsuya Hara, Ahmed Tawakol, Farouc A. Jaffer, Peter K. Henke, Chase W. Kessinger, Gregory R. Wojtkiewicz, Ralph Weissleder, Kimmo Jokivarsi, Anna-Liisa Brownell, William J. Hucker |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Male
medicine.medical_specialty Neutropenia Time Factors Neutrophils Deep vein Computed tomography Multimodal Imaging Sensitivity and Specificity Article Fluorodeoxyglucose positron emission tomography Cohort Studies Mice Fluorodeoxyglucose F18 Recurrence Physiology (medical) Jugular vein medicine Animals Humans cardiovascular diseases Thrombus Ligation Retrospective Studies Venous Thrombosis medicine.diagnostic_test business.industry Thrombosis Middle Aged medicine.disease Mice Inbred C57BL Disease Models Animal medicine.anatomical_structure Case-Control Studies Positron-Emission Tomography Female Radiology Tomography Cardiology and Cardiovascular Medicine business Tomography X-Ray Computed |
| Popis: | Background— Accurate detection of recurrent same-site deep vein thrombosis (DVT) is a challenging clinical problem. Because DVT formation and resolution are associated with a preponderance of inflammatory cells, we investigated whether noninvasive 18 F-fluorodeoxyglucose (FDG)-positron emission tomography (PET) imaging could identify inflamed, recently formed thrombi and thereby improve the diagnosis of recurrent DVT. Methods and Results— We established a stasis-induced DVT model in murine jugular veins and also a novel model of recurrent stasis DVT in mice. C57BL/6 mice (n=35) underwent ligation of the jugular vein to induce stasis DVT. FDG-PET/computed tomography (CT) was performed at DVT time points of day 2, 4, 7, 14, or 2+16 (same-site recurrent DVT at day 2 overlying a primary DVT at day 16). Antibody-based neutrophil depletion was performed in a subset of mice before DVT formation and FDG-PET/CT. In a clinical study, 38 patients with lower extremity DVT or controls undergoing FDG-PET were analyzed. Stasis DVT demonstrated that the highest FDG signal occurred at day 2, followed by a time-dependent decrease ( P P P =0.03). Recurrent DVT demonstrated significantly higher FDG uptake than organized day 14 DVT ( P =0.03). The FDG DVT signal in patients also exhibited a time-dependent decrease ( P Conclusions— Noninvasive FDG-PET/CT identifies neutrophil-dependent thrombus inflammation in murine DVT, and demonstrates a time-dependent signal decrease in both murine and clinical DVT. FDG-PET/CT may offer a molecular imaging strategy to accurately diagnose recurrent DVT. |
| Databáze: | OpenAIRE |
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