The identification of abnormal glycoforms of serum transferrin in carbohydrate deficient glycoprotein syndrome type i by capillary zone electrophoresis
| Autor: | Raymond A. Dwek, Oleg Iourin, Pauline M. Rudd, Nasi Mian, Geoffrey Keir, Bryan Winchester, Taj S. Mattu |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Glycan
Glycosylation Glycoside Hydrolases Population Carbohydrates Neuraminidase Sensitivity and Specificity Biochemistry chemistry.chemical_compound Congenital Disorders of Glycosylation Capillary electrophoresis Polysaccharides Exoglycosidase Humans education Molecular Biology Chromatography High Pressure Liquid chemistry.chemical_classification education.field_of_study biology Transferrin Electrophoresis Capillary Cell Biology beta-Galactosidase N-Acetylneuraminic Acid Sialic acid chemistry biology.protein Glycoprotein |
| Zdroj: | Glycoconjugate Journal. 13:1031-1042 |
| ISSN: | 1573-4986 0282-0080 |
| DOI: | 10.1007/bf01053199 |
| Popis: | One of the biochemical characteristics of carbohydrate deficient glycoprotein syndromes is the presence of abnormal glycoforms in serum transferrin. Both glycoform heterogeneity and variable site occupancy may, in principle, lead to the generation of a range of glycoforms which contain different numbers of sialic acid residues, and therefore variable amounts of negative charge. Capillary zone electrophoresis was used to resolve the glycoforms of normal human serum transferrin and also of a set of glycoforms which were prepared by digesting the sugars on the intact glycoprotein with sialidase. The sugars on the intact glycoprotein were also modified by a series of exoglycosidase enzymes to produce a series of neutral glycoforms which were-also analysed by capillary zone electrophoresis. The oligosaccharide population of human serum transferrin was analysed by a series of mixed exoglycosidase digests on the released glycan pool and quantified using a novel HPLC strategy. Transferrin was isolated from carbohydrate deficient glycoprotein syndromes type I serum and both the intact glycoforms and released sugars were resolved and quantified. The data presented here confirm the presence of a hexa-, penta- and tetra-sialoforms of human serum transferrin in both normal and carbohydrate deficient glycoprotein syndrome type I serum samples. Consistent with previous reports carbohydrate deficient glycoprotein syndrome type I transferrin also contained a di-sialoform, representing a glycoform in which one of the two N-glycosylation sites is unoccupied, and a non-glycosylated form where both remain unoccupied. This study demonstrates that capillary zone electrophoresis can be used to resolve quantitatively both sialylated and neutral complex type glycoforms, suggesting a rapid diagnostic test for the carbohydrate deficient glycoprotein syndromes group of diseases. |
| Databáze: | OpenAIRE |
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