Targeted genome modifications in cereal crops

Autor:	Robert Eric Hoffie, Jochen Kumlehn, Fumitaka Abe, Hiroshi Hisano
Rok vydání:	2021
Předmět:	Transcription activator-like effector nuclease Invited Review rice Effective force barley Plant Science Computational biology Biology maize Genome Zinc finger nuclease TALEN Target site Genome editing CRISPR wheat zinc-finger nucleases Genetics Agronomy and Crop Science Gene
Zdroj:	Breeding Science
ISSN:	1347-3735 1344-7610
DOI:	10.1270/jsbbs.21019
Popis:	The recent advent of customizable endonucleases has led to remarkable advances in genetic engineering, as these molecular scissors allow for the targeted introduction of mutations or even precisely predefined genetic modifications into virtually any genomic target site of choice. Thanks to its unprecedented precision, efficiency, and functional versatility, this technology, commonly referred to as genome editing, has become an effective force not only in basic research devoted to the elucidation of gene function, but also for knowledge-based improvement of crop traits. Among the different platforms currently available for site-directed genome modifications, RNA-guided clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) endonucleases have proven to be the most powerful. This review provides an application-oriented overview of the development of customizable endonucleases, current approaches to cereal crop breeding, and future opportunities in this field.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f282add7705bd223a557be492876b302 https://doi.org/10.1270/jsbbs.21019 Zobrazit plný text záznamu Plný text ve formátu PDF