In vitro and in vivo detection of lactate with nanohybrid-functionalized Pt microelectrode facilitating assessment of tumor development
| Autor: | Fei Xiao, Zheng Wang, Yun Xu, Shuai Wang, Lin Wang, Qiying Lv, Yuji Gong, Huiling Fan, Yan Zhang, Qilin Li, Yunruo Xu |
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| Rok vydání: | 2021 |
| Předmět: |
Biomedical Engineering
Biophysics Metal Nanoparticles Biosensing Techniques 02 engineering and technology 01 natural sciences Limit of Detection In vivo Neoplasms Electrochemistry Extracellular Humans Lactic Acid Detection limit Chemistry 010401 analytical chemistry Electrochemical Techniques Hydrogen Peroxide General Medicine 021001 nanoscience & nanotechnology Small molecule In vitro 0104 chemical sciences Microelectrode Anaerobic glycolysis Tumor progression Gold 0210 nano-technology Microelectrodes Biotechnology |
| Zdroj: | Biosensors and Bioelectronics. 191:113474 |
| ISSN: | 0956-5663 |
| DOI: | 10.1016/j.bios.2021.113474 |
| Popis: | Accelerated glucose uptake and “aerobic glycolysis” of tumor cells generates a high-level lactate in extracellular space and within tumor tissue, which is thought to be a hallmark of tumor and closely correlated with tumor development. Here, we report the development of an enzyme-free electrochemical sensing platform based on a Pt-microneedle electrode functionalized with Au nanoparticles (Au-NPs) decorated polydopamine nanospheres (PDA-NSs), and explore its practical application in in vitro and in vivo detection of lactate in different biological samples. Our results demonstrate that in virtue of the nanostructured merits and high electrocatalytic activity, the resultant nanohybrid-microelectrode exhibits good sensitivity and selectivity to the nonenzymatic electrochemical detection of lactate, with a detection limit of 50 μM, a liner range of 0.375–12 mM, and a sensitivity of 11.25 mA mM−1 cm−2, as well as a good anti-interference ability to other active small molecules. The platform quantifies lactate in complex bio-fluids, including cancerous and non-cancerous cell culture media, as well as serum samples, with detecting time 7.5-fold faster than does a clinically-used approach. Moreover, owing to miniaturized size and satisfactory electrochemical performance, the sensor achieves in vivo recording of lactate-related characteristic voltammetric signals within a living tumor, which are positively correlated with tumor burden and growth. Therefore, the platform cannot only be employed for cancer metabolic investigation, but also potentially for clinical assessment of tumor progression, and even clinical diagnosis of other lactate metabolism disorders. |
| Databáze: | OpenAIRE |
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