The isolation of polyadenylate with unreacted cellulose
| Autor: | P.A. Kitos, Harold Amos, G. Saxon |
|---|---|
| Rok vydání: | 1972 |
| Předmět: |
Uracil Nucleotides
Polynucleotides Biophysics DNA Single-Stranded Cytosine Nucleotides Tritium Biochemistry chemistry.chemical_compound Cytosine nucleotide L Cells Ribonucleases Adenine nucleotide Polyadenylate Thymine Nucleotides RNA Messenger Cellulose Molecular Biology Inosine Nucleotides Chromatography Deoxyribonucleases Adenine Nucleotides Hydrolysis Osmolar Concentration RNA Cell Biology DNA Hydrogen-Ion Concentration chemistry Polynucleotide Uracil nucleotide |
| Zdroj: | Biochemical and biophysical research communications. 47(6) |
| ISSN: | 0006-291X |
| Popis: | Cellulose, unesterified with polydeoxythymidylate, polyuridylate or acetate selectively binds polyadenylate (poly [A]) and poly [A]- containing RNAs in high salt solution or in low salt solution at pH values of 5.5 or less. It does not bind heteropolymeric RNA but does have some affinity for DNA, particularly in its single-stranded form. These binding properties are useful in the isolation of heterogeneous nuclear and messenger RNAs of eukaryotic cells for analytic and preparative purposes. |
| Databáze: | OpenAIRE |
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