Rhodopsin′s Secondary Structure Revisited: Assignment of Structural Elements
| Autor: | W. J. De Grip, Arthur M.A. Pistorius |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Rhodopsin
biology Chemistry Serine Endopeptidases Thermolysin Biophysics Analytical chemistry Photoprotein Infrared spectroscopy Cell Biology Rod Cell Outer Segment Proteinase K Biochemistry Protein Structure Secondary Crystallography Membrane Protein structure Spectroscopy Fourier Transform Infrared biology.protein sense organs Endopeptidase K Molecular Biology Protein secondary structure |
| Zdroj: | Biochemical and Biophysical Research Communications. 198:1040-1045 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.1994.1148 |
| Popis: | FT-IR spectroscopy has been applied to study the secondary structure of rhodopsin in dehydrated films of bovine rod photoreceptor membranes. Curve fitting analysis of the amide I band around 1658 cm-1 compares well with data obtained from samples in the hydrated state. Repeating this analysis on samples, treated with proteinase K or thermolysin, secondary structural elements at the cytoplasmic side of the photoreceptor membrane can be located. We present evidence for the location of a beta-sheet and a beta-turn near the lipid anchor in the C-terminal region of the protein, and for an alpha-helical structure in the third cytoplasmic loop. |
| Databáze: | OpenAIRE |
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