Absence of High-Affinity Binding Sites for Interferon α/β in Variant Murine CD4+T Lymphocytes Not Expressing the T Cell Antigen Receptor
| Autor: | Connie R. Faltynek, M. Tarek Shata, Roberta Kamin-Lewis, George K. Lewis |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
CD4-Positive T-Lymphocytes
Mice Inbred A T cell Immunology T-cell receptor B-cell receptor Receptors Antigen T-Cell CD28 Cell Biology Biology Molecular biology Cell Line Mice medicine.anatomical_structure Reference Values Virology Interferon Type I medicine Animals Cytotoxic T cell IL-2 receptor Antigen-presenting cell CD8 Receptors Interferon |
| Zdroj: | Journal of Interferon & Cytokine Research. 15:291-296 |
| ISSN: | 1557-7465 1079-9907 |
| DOI: | 10.1089/jir.1995.15.291 |
| Popis: | The T cell antigen receptor complex (CD3/Ti) plays a role in specific antigen recognition as well as in signal transduction, with its surface expression required for the function of several other structurally distinct receptor systems, including CD2, Ly-6(TAP), and Thy-1. In this communication, evidence is presented suggesting an association between the surface expression of CD3/Ti and that of the type 1 interferon (IFN) receptor in a CD4+ murine T cell clone. We tested the proliferative responses and their capacity to be inhibited by type 1 IFN with the wild-type, CD3/Ti-positive T cell clone and its CD3/Ti-negative variants did not respond to specific antigen or anti-CD3 antibody stimulation but they did respond to T cell growth factor (TCGF), stimulation as did the wild-type parental cells. Therefore, the type 1 IFN inhibition of TCGF-stimulated proliferative responses of wild-type and variant cells were compared. Both natural and recombinant type 1 IFNs inhibited TCGF-induced tritiated thymidine (3H-TdR) incorporation in the wild-type T cell clone, with a ID50 of 60-80 U/ml. By contrast, the variants required much higher doses of type 1 IFN. The ID50 with natural murine IFN-beta was 10,000 U/ml, but this same dose of human IFN-alpha A/D gave only a marginal inhibitory effect. Accompanying the loss of IFN responsiveness, these variants also exhibited a loss of high-affinity type 1 IFN receptors. Taken together, these data suggest that the CD3/Ti complex plays a role in the surface expression of the type 1 IFN receptor in a CD4+ T cell clone.(ABSTRACT TRUNCATED AT 250 WORDS) |
| Databáze: | OpenAIRE |
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