Leucine-responsive regulatory protein, IS1 insertions, and the negative regulator FaeA control the expression of the fae (K88) operon in Escherichia coli
| Autor: | F K de Graaf, T T Huisman, P Klaasen, D. Bakker |
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| Rok vydání: | 1994 |
| Předmět: |
DNA
Bacterial Operon Fimbria Molecular Sequence Data Biology Microbiology Fimbriae Proteins Bacterial Proteins Sequence Homology Nucleic Acid Genes Regulator Leucine-responsive regulatory protein Escherichia coli Amino Acid Sequence Cloning Molecular Promoter Regions Genetic Molecular Biology Peptide sequence Regulator gene Genetics Regulation of gene expression Antigens Bacterial Base Sequence Sequence Homology Amino Acid Escherichia coli Proteins Gene Expression Regulation Bacterial biochemical phenomena metabolism and nutrition Leucine-Responsive Regulatory Protein female genital diseases and pregnancy complications DNA-Binding Proteins Repressor Proteins Regulatory sequence Antigens Surface biology.protein DNA Transposable Elements bacteria Transcription Factors |
| Zdroj: | Molecular microbiology. 11(3) |
| ISSN: | 0950-382X |
| Popis: | Nucleotide sequence analysis of the fae operon encoding the biosynthesis of K88 fimbriae revealed the presence of two divergently transcribed regulatory genes, faeA and faeB, separated by two inverted IS1 insertions. The amino acid sequences of the regulatory proteins FaeA and FaeB show similarity to the primary structure of corresponding regulatory proteins involved in the biosynthesis of Pap and S fimbriae. Expression of faeA is positively controlled by the FaeA protein, whereas K88 fimbriae production is negatively controlled by the co-operative activity of FaeA and the leucine-responsive regulatory protein (Lrp). Exchange of FaeA for Papl, a positive regulator of Pap fimbriae expression, also represses K88 production indicating that the combination Papl/Lrp has opposite effects on fae and pap expression. Mutations in faeB had no effect on the biosynthesis of K88 fimbriae. The presence of the two IS1 insertions is hypothesized to neutralize part of the repression of K88 biosynthesis by FaeA/Lrp. Like pap, the fae operon does not respond to exogenous leucine. |
| Databáze: | OpenAIRE |
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