Effect of strontium surface-functionalized implants on early and late osseointegration: A histological, spectrometric and tomographic evaluation

Autor: Bernd Lethaus, Caroline Öhman-Mägi, M. Sillassen, Ole Zoffmann Andersen, Frank Kloss, Morten Foss, Christian Sloth Jeppesen, Heribert Talasz, Vincent Offermanns, K.P. Almtoft, Gregor Riede, Rene Tolba
Rok vydání: 2018
Předmět:
Male
medicine.medical_treatment
02 engineering and technology
OSTEOPOROSIS
Biochemistry
Annan materialteknik
0302 clinical medicine
Osteogenesis
Femur
Dental implant
COATINGS
Titanium
Bone growth
Bone-Anchored Prosthesis
TOTAL HIP-REPLACEMENT
General Medicine
021001 nanoscience & nanotechnology
FRACTURE
DENTAL IMPLANTS
Rabbits
BONE
0210 nano-technology
Biotechnology
RANELATE
Biomaterialvetenskap
Biomedical Engineering
chemistry.chemical_element
Biofunctionalization
OSTEOCONDUCTIVITY
Osseointegration
Biomaterials
03 medical and health sciences
medicine
Animals
Other Materials Engineering
Nanotopography
Molecular Biology
Strontium
Orthopedic
X-Ray Microtomography
030206 dentistry
NANOTOPOGRAPHY
TITANIUM IMPLANTS
Osteoinduction
chemistry
Release
Biomaterials Science
Dental
Surface modification
Implant
Biomedical engineering
Zdroj: Offermanns, V, Andersen, O Z, Riede, G, Sillassen, M, Jeppesen, C S, Almtoft, K P, Talasz, H, Ohman-Magi, C, Lethaus, B, Tolba, R, Kloss, F & Foss, M 2018, ' Effect of strontium surface-functionalized implants on early and late osseointegration : A histological, spectrometric and tomographic evaluation ', Acta Biomaterialia, vol. 69, pp. 385-394 . https://doi.org/10.1016/j.actbio.2018.01.049
ISSN: 1742-7061
DOI: 10.1016/j.actbio.2018.01.049
Popis: Numerous in vivo, in vitro and clinical studies report on beneficial effects of strontium with respect to increased bone growth. Based on this knowledge the aim of this study was to evaluate early and late osseointegration stages of functionalized titanium implants showing sustained release of strontium (Sr) and further investigate its potential systemic effect. Strontium functionalized (Ti-Sr-O) and Grade 4 (Control) titanium implants were inserted in the femoral condyle of New Zealand White rabbits. The Ti-Sr-O coating was characterized using Scanning Electron Microscopy (SEM) and Energy Dispersive X-ray Spectrometry (EDX) for structure, coating thickness and chemical composition. Inductively Coupled Plasma Atomic Emission Spectrometry (ICP-AES) was used to evaluate released strontium in vitro while Atomic Absorption Spectrometry (AAS) was utilized to monitor serum levels of strontium and calcium. Additionally, histological and tomographic analysis of bone-to-implant contact (BIC%) and bone formation (BF%) was performed, following implantation periods of two or twelve weeks, respectively. Median values for BIC% for Ti-Sr-O revealed significant differences within the two-and twelve-week observation periods, while exceeding BF% was discovered especially after twelve weeks when performing the histological evaluation. The results from the micro-computed tomography (mu-CT) showed no significant differences, when comparing the experimental groups. AAS measurements did not indicate a systemic effect by the local strontium release. Within the limitations of the study, it was shown that a Ti-Sr-O coating with sustained release characteristics of strontium, accelerates bone apposition and represents a potential potent surface modification for endosseous medical implant devices.Statement of SignificanceThis study presents first data with respect to early and late in vivo response on a strontium functionalized titanium surface comprising a nanotopography manufactured by a magnetron sputtering process. We investigated different osseointegration stages of screw-shaped implants with dental implant geometries in a rabbit femur model observing beneficial effects of the functionalized surface on bone-to-implant contact and bone formation caused by tailored release of the bone anabolic strontium. Histomorphometrical data revealed that a functionalized titanium surface with controlled liberation of strontium accelerates osseointegration while spectrometry measurements did not indicate a potential systemic effect of this osteoinductive agent and could thus have impact on modifications of medical implant devices. (C) 2018 Acta Materialia Inc. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND.
Databáze: OpenAIRE
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