Recent developments in microarray-based enzyme assays: from functional annotation to substrate/inhibitor fingerprinting

Autor:	Hongyan Sun, Jun Wang, Shao Q. Yao, Souvik Chattopadhaya
Rok vydání:	2006
Předmět:	Microarray Substrate (chemistry) Biology Microarray Analysis Proteomics Biochemistry Enzymes Substrate Specificity Analytical Chemistry Functional annotation Animals Humans Enzyme Inhibitors DNA microarray
Zdroj:	Analytical and Bioanalytical Chemistry. 386:416-426
ISSN:	1618-2650 1618-2642
Popis:	Recent advances in proteomics have provided impetus towards the development of robust technologies for high-throughput studies of enzymes. The term "catalomics" defines an emerging '-omics' field in which high-throughput studies of enzymes are carried out by using advanced chemical proteomics approaches. Of the various available methods, microarrays have emerged as a powerful and versatile platform to accelerate not only the functional annotation but also the substrate and inhibitor specificity (e.g. substrate and inhibitor fingerprinting, respectively) of enzymes. Herein, we review recent developments in the fabrication of various types of microarray technologies (protein-, peptide- and small-molecule-based microarrays) and their applications in high-throughput characterizations of enzymes.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f27637594cd98602658cb5dbd57027b1 https://doi.org/10.1007/s00216-006-0511-5 Zobrazit plný text záznamu Plný text ve formátu PDF