Characterization complex collagen fiber architecture in knee joint using high‐resolution diffusion imaging
Autor: | Nian Wang, Matthew J. Hilton, Yi Qi, Gary P. Cofer, G. Allan Johnson, Anthony J. Mirando |
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Rok vydání: | 2020 |
Předmět: |
Cartilage
Articular musculoskeletal diseases Materials science Knee Joint Meniscus (anatomy) Article 030218 nuclear medicine & medical imaging 03 medical and health sciences 0302 clinical medicine Image Processing Computer-Assisted medicine Animals Radiology Nuclear Medicine and imaging Fiber Cartilage musculoskeletal system Rats Diffusion Magnetic Resonance Imaging Diffusion Tensor Imaging medicine.anatomical_structure Ligament Spin echo Collagen 030217 neurology & neurosurgery Biomedical engineering Diffusion MRI Tractography |
Zdroj: | Magn Reson Med |
ISSN: | 1522-2594 0740-3194 |
Popis: | PURPOSE: To evaluate the complex fiber orientations and three-dimensional collagen fiber network of knee joint connective tissues, including ligaments, muscle, articular cartilage, and meniscus using high spatial and angular resolution diffusion imaging. METHODS: Two rat knee joints were scanned using a modified 3D diffusion-weighted spin echo pulse sequence with the isotropic spatial resolution of 45 μm at 9.4T. The b values varied from 250 to 1250 s/mm(2) with 31 diffusion encoding directions for one rat knee. The b value was fixed to 1000 s/mm(2) with 147 diffusion encoding directions for the second knee. Both diffusion tensor imaging (DTI) model and generalized Q-sampling imaging (GQI) method were used to investigate the fiber orientation distributions and tractography with the validation of polarized light microscopy (PLM). RESULTS: In order to better resolve the crossing fibers, the b value should be great than or equal to 1000 s/mm(2). The tractography results were comparable between DTI model and GQI method in ligament and muscle. However, the tractography exhibited apparent difference between DTI and GQI in connective tissues with more complex collagen fibers network, such as cartilage and meniscus. In articular cartilage, there were numerous crossing fibers found in superficial zone (SZ) and transitional zone (TZ). Tractography generated with GQI also resulted in more intact tracts in articular cartilage than DTI. CONCLUSION: High resolution diffusion imaging with GQI method can trace the complex collagen fiber orientations and architectures of the knee joint at microscopic resolution. |
Databáze: | OpenAIRE |
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