Nuclear-magnetic-resonance studies of human epidermal growth factor
| Autor: | Michael J. Tappin, Tatsuo Miyazawa, Daisuke Kohda, Robert M. Cooke, Tetsuo Miyake, Iain D. Campbell, Fuyuhiko Inagaki, Toru Fuwa |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Magnetic Resonance Spectroscopy
Epidermal Growth Factor Chemistry Human epidermal growth factor Protein Conformation Recombinant Fusion Proteins Molecular Sequence Data Nuclear Overhauser effect Biochemistry Rats Mice Nuclear magnetic resonance Nerve growth factor Sequence Homology Nucleic Acid Animals Humans Amino Acid Sequence Transforming growth factor Hydrogen |
| Zdroj: | European journal of biochemistry. 193(3) |
| ISSN: | 0014-2956 |
| Popis: | The 1H-NMR spectra of native human epidermal growth factor (EGF) and a derivative lacking the final five residues have been assigned by two-dimensional methods, enabling their structures to be compared. The same structural features are observed for each protein, although the final five residues of native human EGF interact with residues earlier in the sequence. Comparison of the resonance shifts of human, rat and mouse EGF and human transforming growth factor alpha (TGF alpha) enables shifts characteristic of the EGF conformation to be identified, providing standards by which the structures of related proteins may be assessed. |
| Databáze: | OpenAIRE |
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