Ex Vivo Model of Functional Mitral Regurgitation Using Deer Hearts

Autor: Emiliano Votta, Riccardo Vismara, Federico Lucherini, Gianfranco Beniamino Fiore, Eleonora Salurso, Edoardo Maroncelli, Carlo Antona, Andrea Mangini, Rubina Rosa, Michal Jaworek
Rok vydání: 2020
Předmět:
medicine.medical_specialty
0206 medical engineering
Echocardiography
Three-Dimensional
Pulsatile flow
Pharmaceutical Science
02 engineering and technology
030204 cardiovascular system & hematology
Ventricular Function
Left
Heart mitral valve
03 medical and health sciences
Preclinical research
Beating heart model
0302 clinical medicine
Mitral valve
Internal medicine
Genetics
Animals
Fiber Optic Technology
Medicine
cardiovascular diseases
Mitral annulus
Systole
Atrial mitral regurgitation
Functional mitral regurgitation
Genetics (clinical)
business.industry
Deer
Heart valve pathological model
Hemodynamics
Ischemic mitral regurgitation
Mitral Valve Insufficiency
Isolated Heart Preparation
020601 biomedical engineering
Ex vivo
Disease Models
Animal
medicine.anatomical_structure
cardiovascular system
Cardiology
Molecular Medicine
Original Article
Cardiology and Cardiovascular Medicine
business
Zdroj: Journal of Cardiovascular Translational Research
ISSN: 1937-5395
1937-5387
DOI: 10.1007/s12265-020-10071-y
Popis: Transcatheter therapies are emerging for functional mitral regurgitation (FMR) treatment, however there is lack of pathological models for their preclinical assessment. We investigated the applicability of deer hearts for this purpose. 8 whole deer hearts were housed in a pulsatile flow bench. At baseline, all mitral valves featured normal coaptation. The pathological state was induced by 60-minutes intraventricular constant pressurization. It caused mitral annulus dilation (antero-posterior diameter increase from 31.8 ± 5.6 mm to 39.5 ± 4.9 mm, p = 0.001), leaflets tethering (maximal tenting height increase from 7.3 ± 2.5 mm to 12.7 ± 3.4 mm, p < 0.001) and left ventricular diameter increase (from 67.8 ± 7.5 mm to 79.4 ± 6.5 mm, p = 0.004). These geometrical reconfigurations led to restricted mitral valve leaflets motion and leaflet coaptation loss. Preliminary feasibility assessment of two FMR treatments was performed in the developed model. Deer hearts showed ability to dilate under constant pressurization and have potential to be used for realistic preclinical research of novel FMR therapies. Graphical abstract figure legend: Deer heart mitral valve fiberscopic and echocardiographic images in peak systole at baseline and after inducing the pathological conditions representing functional mitral regurgitation. In the pathological conditions lack of coaptation between the leaflets, enlargement of the antero-posterior distance (red dashed line) and the left ventricular diameter (orange dashed line) were observed. Electronic supplementary material The online version of this article (10.1007/s12265-020-10071-y) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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