Increased expression of C5a receptor (CD88) mRNA in canine mammary tumors
| Autor: | Mohd Noor Mohd Hezmee, Barbara E. Rolfe, M.T. Kyaw-Tanner, Ian A. Shiels, Paul C. Mills, Jia Yu Peppermint Lee, Trent M. Woodruff |
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| Rok vydání: | 2011 |
| Předmět: |
Pathology
medicine.medical_specialty Myoepithelioma Immunology Mammary Neoplasms Animal Inflammation Biology C5a receptor Dogs Mammary Glands Animal Breast cancer medicine Animals Dog Diseases RNA Messenger Receptor Receptor Anaphylatoxin C5a Messenger RNA Mammary tumor General Veterinary Reverse Transcriptase Polymerase Chain Reaction Carcinoma medicine.disease Complement system Gene Expression Regulation Neoplastic Female medicine.symptom |
| Zdroj: | Veterinary Immunology and Immunopathology. 139:50-56 |
| ISSN: | 0165-2427 |
| DOI: | 10.1016/j.vetimm.2010.08.005 |
| Popis: | Mammary tumors are among the most common neoplastic conditions in dogs, and there is evidence that inflammation plays a role in the development of some tumor types in dogs. The complement system is a major participant in the inflammatory process and the complement activation component, C5a, is a potent inflammatory peptide. This study investigated the mRNA expression of the major receptor for C5a (C5aR; CD88) in histopathological samples of canine mammary tumors by quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) using canine-specific primers for CD88. A total of seven canine mammary tumors (four malignant carcinomas, two benign mixed mammary tumors, and one myoepithelioma) and eight normal mammary glands were analysed. All the tumor samples expressed low levels of CD88 mRNA, while none of the normal mammary tissues showed any detectable expression. These preliminary results suggest that C5a-CD88 interaction may play a contributory role in the inflammatory response associated with mammary tumor development in dogs. Further studies investigating the mechanisms behind complement activation and C5a receptor expression in canine mammary tumors are warranted. |
| Databáze: | OpenAIRE |
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