Real-time MR imaging of adeno-associated viral vector delivery to the primate brain
| Autor: | Phillip Pivirotto, Janine Beyer, Massimo S. Fiandaca, Jamie L. Eberling, Howard J. Federoff, Piotr Hadaczek, John Bringas, John Forsayeth, Krystof S. Bankiewicz, John W. Park, William J. Bowers, Tracy R. McKnight, Vanja Varenika |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Male
Pathology medicine.medical_specialty viruses Cognitive Neuroscience Genetic Vectors Green Fluorescent Proteins Gadolinium Biology medicine.disease_cause Article Viral vector Thalamus In vivo medicine Animals Humans Distribution (pharmacology) Adeno-associated virus medicine.diagnostic_test Putamen Gene Transfer Techniques Brain Magnetic resonance imaging Dependovirus Immunohistochemistry Macaca mulatta Magnetic Resonance Imaging Neurology Aromatic-L-Amino-Acid Decarboxylases Positron emission tomography Positron-Emission Tomography Liposomes |
| Zdroj: | NeuroImage. 47:T27-T35 |
| ISSN: | 1053-8119 |
| DOI: | 10.1016/j.neuroimage.2008.11.012 |
| Popis: | We are developing a method for real-time magnetic resonance imaging (MRI) visualization of convection-enhanced delivery (CED) of adeno-associated viral vectors (AAV) to the primate brain. By including gadolinium-loaded liposomes (GDL) with AAV, we can track the convective movement of viral particles by continuous monitoring of distribution of surrogate GDL. In order to validate this approach, we infused two AAV (AAV1-GFP and AAV2-hAADC) into three different regions of non-human primate brain (corona radiata, putamen, and thalamus). The procedure was tolerated well by all three animals in the study. The distribution of GFP determined by immunohistochemistry in both brain regions correlated closely with distribution of GDL determined by MRI. Co-distribution was weaker with AAV2-hAADC, although in vivo PET scanning with FMT for AADC activity correlated well with immunohistochemistry of AADC. Although this is a relatively small study, it appears that AAV1 correlates better with MRI-monitored delivery than does AAV2. It seems likely that the difference in distribution may be due to differences in tissue specificity of the two serotypes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect